Construction of mRNA Regulatory Networks Reveals the Key Genes in Atrial Fibrillation

Comput. Math. Methods Medicine Pub Date : 2021-05-24 DOI:10.1155/2021/5527240

Nannan Chen, Mu Qin, Qunlin Gong, Nan Xu, Yu Lin, Jiahong Wang, Pengxiang Zheng

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Abstract

Atrial fibrillation (AF), the most familiar heart rhythm disorder, is a major cause of stroke in the world, whereas the mechanism behind AF remains largely unclear. In the current study, we used the RNA-seq method to identify 275 positively regulated mRNAs and 117 negatively regulated mRNAs in AF compared to healthy controls. Through bioinformatic analysis, it indicated that these distinctively expressed genes took part in regulating multiple AF-related biological processes and pathways, such as platelet aggregation, platelet activation, pri-miRNA transcription, and transforming growth factor-beta (TGF-β) receptor signaling pathway. Protein-protein interaction (PPI) network analysis identified ITGB5, SRC, ACTG1, ILK, ITGA2B, ITGB3, TUBB4B, CDK11A, PAFAH1B1, CDK11B, and TUBG1 as hub regulators in AF. Moreover, the quantitative real-time PCR (qRT-PCR) assay was conducted and revealed that these hub genes were remarkably overexpressed in AF samples compared to normal samples. We believed that this study would enrich the understanding of the pathogenesis of AF and enable further research on the pathogenesis of AF.

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mRNA调控网络的构建揭示心房颤动的关键基因

房颤(AF)是世界上最常见的心律失常，是导致中风的主要原因，但其发病机制仍不清楚。在目前的研究中，我们使用RNA-seq方法鉴定了与健康对照相比，AF中275个正调控mrna和117个负调控mrna。生物信息学分析表明，这些特异性表达基因参与调控多种af相关的生物学过程和途径，如血小板聚集、血小板活化、pri-miRNA转录、转化生长因子-β (TGF-β)受体信号通路。蛋白-蛋白相互作用(PPI)网络分析鉴定出ITGB5、SRC、ACTG1、ILK、ITGA2B、ITGB3、TUBB4B、CDK11A、PAFAH1B1、CDK11B和TUBG1是AF中的枢纽调节因子。此外，通过实时荧光定量PCR (qRT-PCR)检测发现，这些枢纽基因在AF中明显过表达。我们相信本研究将丰富对房颤发病机制的认识，为房颤发病机制的进一步研究奠定基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Comput. Math. Methods Medicine

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