{"title":"[GTP-dependent dopamine reception in central nervous system tissues in the pond snail].","authors":"I M Prudnikov, V N Tsyvkin, T F Kastrikina","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Guanosine-5'-triphosphate (GTP)-dependent binding of the agonist D1 dopamine (DA) receptor ligand [H3]-SKF 38393 is described. It is shown that binding of [H3]-SKF 38393 with two different populations takes place in the presence of guanylyl nucleotides, when they are absent--with one population. It is demonstrated using GDP-alpha-P33 binding analysis that the GDP in equilibrium with GTP exchange rate gets higher as a result of activation of DA receptors in the membrane from the mollusc nervous tissues. Influence of the catalytic subunit of the protein kinase A (cPKA) on the [H3]-SKF 38393 and GDP-alpha-P33 is investigated. Obvious influence of the cPKA on [H3]-SKF 38393 binding is not observed, while basal and DA-induced GDP in equilibrium with GTP exchange in membranes of the mollusc nervous tissues is considerably inhibited.</p>","PeriodicalId":19121,"journal":{"name":"Neirofiziologiia = Neurophysiology","volume":"24 4","pages":"451-61"},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neirofiziologiia = Neurophysiology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Guanosine-5'-triphosphate (GTP)-dependent binding of the agonist D1 dopamine (DA) receptor ligand [H3]-SKF 38393 is described. It is shown that binding of [H3]-SKF 38393 with two different populations takes place in the presence of guanylyl nucleotides, when they are absent--with one population. It is demonstrated using GDP-alpha-P33 binding analysis that the GDP in equilibrium with GTP exchange rate gets higher as a result of activation of DA receptors in the membrane from the mollusc nervous tissues. Influence of the catalytic subunit of the protein kinase A (cPKA) on the [H3]-SKF 38393 and GDP-alpha-P33 is investigated. Obvious influence of the cPKA on [H3]-SKF 38393 binding is not observed, while basal and DA-induced GDP in equilibrium with GTP exchange in membranes of the mollusc nervous tissues is considerably inhibited.
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