Bioethanol Production from Spent Shiitake (Lentinula edodes) Mushroom Medium by Oxalic Acid Pretreatment

So-Yeon Jeong, Jae-Won Lee
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Abstract

In this study, oxalic acid pretreatment of spent mushroom medium was performed depending on combined severity factor (CSF) to produce bioethanol. Glucose was the most abundant compound in the hydrolysate. The degradation rate of biomass was gradually increased by increasing the CSF during pretreatment. The highest ethanol production from hydrolysate was 2.79 g/L at CSF 1.89 (30 min, 82 mM oxalic acid) after 72 h fermentation. The fermentation inhibitor concentration increased by increasing the CSF. In the simultaneous saccharification and fermentation (SSF), the highest ethanol yield was 35.86% at CSF 2.18 (58 min, 82 mM oxalic acid). The optimal pretreatment condition for bioethanol production from the hydrolysate and pretreated biomass was CSF 1.89 (8.64 g/L).
草酸预处理废香菇培养基生产生物乙醇
在本研究中,草酸预处理废蘑菇培养基,根据联合严重因子(CSF)生产生物乙醇。葡萄糖是水解物中含量最多的化合物。预处理过程中,随着CSF的增加,生物质的降解率逐渐提高。发酵72 h后,在CSF 1.89 (30 min, 82 mM草酸)条件下,水解产物乙醇产量最高,为2.79 g/L。发酵抑制剂浓度随CSF的增加而增加。同时糖化发酵(SSF)中,在CSF 2.18 (58 min, 82 mM草酸)条件下乙醇产量最高,为35.86%。水解产物和预处理生物质制备生物乙醇的最佳预处理条件为CSF 1.89 (8.64 g/L)。
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