Evaluation of Disease Index, Colon Damage Score, and Microbial Analysis of Intestinal Flora after Treatment with the Novel Test Formulation in TNBS-Induced Colitis in Sprague Dawley Rats

Abstract

The aim of the present study was to evaluate the microbial analysis, Disease Activity/Activation Index (DAI), and Colon Mucosal/Macroscopic Damage Index (CMDI) analysis after treatment with the Biofield Energy Healing/Blessing (the Trivedi Effect ® ) based novel test formulation in male The test formulation consists of minerals (zinc, magnesium, iron, and copper) and vitamins (B 6 , B 12 , and D 3 ), which was divided into two parts. One part was denoted as the control, without any Biofield Energy Healing/Blessing Treatment, while the other part was defined as the Biofield Energy Treated/Blessed sample, which received the Biofield Energy Healing Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Additionally, three group of animals were also received Biofield Energy Treatment per se (day -15) under similar conditions. The gut health microbial analysis along with DAI and CMDI with the help of Elevated Plus Maze (EPM) was evaluated. The DAI score was decreased by 18.8%, 13.5%, 18.6%, 25.5%, and 15.8% in the G5 (Biofield Energy Treated test formulation), G6 (Biofield treatment per se to animals at -15 days), G7 (Biofield Energy Treated test formulation at -15 day), G8 (Biofield Treatment per se to the animals plus Biofield Treated test formulation at -15 day), and G9 (Biofield Treatment per se to the animals plus untreated test formulation) groups, respectively as compared with the G2. EPM data showed a significant increase time spent in open arm by 436.55%, 493.31%, 526.77% ( p ≤0.05), 438.61%, and 133.20% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Microbial analysis showed decrease in the count of coli in the G5, G6, and G8 groups, while a decrease in the Klebsiella pneumonia count was also observed in at 4 th week in all treated groups except the G5 group as compared with the G4. On the other hand, the increase of the beneficial bacteria Bacteriodes uniformis and Prevotella copra was observed in the G5 group. CMDI score was significantly reduced by 19.67%, 13.79%, 25.55% ( p ≤0.05), 29.47% ( p ≤0.05), and 21.63% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. Overall, the data indicated that the Trivedi Effect ® -Biofield Energy Healing based test formulation and Biofield Energy Treatment se reduced the colon inflammation in the TNBS-induced colitis rats by decreasing CMDI and increased DAI along with improved gut microbial analysis.