The Potentials of Robusta Coffee Seed Extract as Antioxidant on Kidney Histopathology in Mice Exposed to Monosodium Glutamate

Feby Dwi Wisudawati, Nove Hidajati, F. Fikri, K. P. Santoso, A. L. Saputro, M. T. E. Purnama
{"title":"The Potentials of Robusta Coffee Seed Extract as Antioxidant on Kidney Histopathology in Mice Exposed to Monosodium Glutamate","authors":"Feby Dwi Wisudawati, Nove Hidajati, F. Fikri, K. P. Santoso, A. L. Saputro, M. T. E. Purnama","doi":"10.21776/ub.vetbioclinj.2023.005.01.3","DOIUrl":null,"url":null,"abstract":"The purpose of this study was to determine the potential of administration robusta coffee extract as an antioxidant on the renal histopathology in mice exposed to monosodium glutamate (MSG) orally. This study used 20 adult male rats with Balb C strain divided into 5 experimental groups, namely K + (MSG 0.12 mg / head and Vitamin C 6 mg/mice), K- (MSG 0.12 mg/mice and Sodium carboxymethyl cellulose (CMC Na) 0,1 ml/mice), P1 (MSG 0.12 mg/mice and Robusta coffee extract 0.1 mg/mice), P2 (MSG 0.12 mg/mice and Robusta coffee extract 0.2 mg/mice), and P3 (MSG 0.12 mg/mice and 0.4 mg Robusta coffee extract mg/mice). The experimental group was treated every day at intervals between MSG and Vitamin C, CMC Na, and Robusta coffee extract for 60 min, the treatment was carried out for 42 days. Kidney histopathology scores were analyzed using the Saphiro-Wilk and Levene test to evaluate homogeneity, followed by the Kruskal Wallis test. The results of the Saphiro Wilk and Levene statistical analysis obtained p<0.05, thus the data continued with the Kruskal Wallis test scores of kidney cell damage obtained 0.043 which showed that the data were significant. The mean score of kidney cell damage in the treatment groups was K+ 1.60; K- 1.63; P1 1.70; P2 1.63; and P3 1.73. The conclusion is that Robusta coffee bean extract did not show a decrease in kidney cell damage that was different between treatment groups. Further research is needed to find out about the effect of different dosages of Robusta Coffee Extract to different organs","PeriodicalId":432813,"journal":{"name":"Veterinary Biomedical and Clinical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Biomedical and Clinical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21776/ub.vetbioclinj.2023.005.01.3","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The purpose of this study was to determine the potential of administration robusta coffee extract as an antioxidant on the renal histopathology in mice exposed to monosodium glutamate (MSG) orally. This study used 20 adult male rats with Balb C strain divided into 5 experimental groups, namely K + (MSG 0.12 mg / head and Vitamin C 6 mg/mice), K- (MSG 0.12 mg/mice and Sodium carboxymethyl cellulose (CMC Na) 0,1 ml/mice), P1 (MSG 0.12 mg/mice and Robusta coffee extract 0.1 mg/mice), P2 (MSG 0.12 mg/mice and Robusta coffee extract 0.2 mg/mice), and P3 (MSG 0.12 mg/mice and 0.4 mg Robusta coffee extract mg/mice). The experimental group was treated every day at intervals between MSG and Vitamin C, CMC Na, and Robusta coffee extract for 60 min, the treatment was carried out for 42 days. Kidney histopathology scores were analyzed using the Saphiro-Wilk and Levene test to evaluate homogeneity, followed by the Kruskal Wallis test. The results of the Saphiro Wilk and Levene statistical analysis obtained p<0.05, thus the data continued with the Kruskal Wallis test scores of kidney cell damage obtained 0.043 which showed that the data were significant. The mean score of kidney cell damage in the treatment groups was K+ 1.60; K- 1.63; P1 1.70; P2 1.63; and P3 1.73. The conclusion is that Robusta coffee bean extract did not show a decrease in kidney cell damage that was different between treatment groups. Further research is needed to find out about the effect of different dosages of Robusta Coffee Extract to different organs
罗布斯塔咖啡种子提取物对谷氨酸钠暴露小鼠肾脏组织病理学的抗氧化作用
本研究的目的是确定罗布斯塔咖啡提取物作为抗氧化剂对口服味精小鼠肾脏组织病理学的影响。本研究选用Balb C品系成年雄性大鼠20只,分为5个实验组,分别为K +(味精0.12 mg/头,维生素C 6 mg/只)、K-(味精0.12 mg/只,羧甲基纤维素钠0.1 ml/只)、P1(味精0.12 mg/只,罗布斯塔咖啡提取物0.1 mg/只)、P2(味精0.12 mg/只,罗布斯塔咖啡提取物0.2 mg/只)、P3(味精0.12 mg/只,罗布斯塔咖啡提取物0.4 mg/只)。试验组每天在味精与维生素C、CMC Na、罗布斯塔咖啡提取物之间交替处理60 min,共处理42 d。采用saphiroo - wilk和Levene检验对肾脏组织病理学评分进行分析,以评估同质性,随后采用Kruskal Wallis检验。sapiro Wilk和Levene统计分析的结果p<0.05,因此数据继续,肾细胞损伤的Kruskal Wallis测试得分为0.043,表明数据有显著性。各治疗组肾细胞损伤平均评分为K+ 1.60;K - 1.63;P1 1.70;P2 1.63;P3 1.73。结论是罗布斯塔咖啡豆提取物并没有显示出肾细胞损伤的减少在不同的治疗组之间是不同的。不同剂量的罗布斯塔咖啡提取物对不同器官的影响需要进一步的研究
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信