In vitro propagation of sacha inchi through organogenesis

R. Solís, D. Cachique, J. C. Guerrero-Abad, María Sánchez, Lourdes Tapia y Figueroa
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引用次数: 8

Abstract

Abstract: The objective of this work was to evaluate hormonal balance in the phases of the in vitro organogenesis of apical meristems of sacha inchi (Plukenetia volubilis). For the establishment and multiplication phases, Murashige & Skoog (MS) media, with different concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), were used. For rooting, modified MS media was supplemented with different concentrations of NAA and indolebutyric acid. The differentiation of apical meristems was possible with 0.1 mg L-1 BAP and 0.05 mg L-1 NAA. The best rooting of seedlings is obtained with 0.5 mg L-1 naphthaleneacetic acid and 2.0 mg L-1 indolebutyric acid.
柞蚕体外器官发生繁殖的研究
摘要/ Abstract摘要:本研究旨在研究印度树(Plukenetia volubilis)顶端分生组织体外器官发生过程中激素平衡的变化。建立期和增殖期采用含不同浓度的苄氨基嘌呤(BAP)和萘乙酸(NAA)的Murashige & Skoog培养基。为了生根,在改良的MS培养基中添加不同浓度的NAA和吲哚丁酸。0.1 mg L-1 BAP和0.05 mg L-1 NAA均可促进根尖分生组织的分化。0.5 mg L-1萘乙酸和2.0 mg L-1吲哚丁酸对幼苗生根效果最好。
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