Impact of Magnesium Oxide Nanoparticles on Hematological, Biochemical and Antioxidant Levels of Mrigal Cirrhinus mrigala

Abstract

Magnesium oxide nanoparticles were synthesized and characterized by using UV-Visible spectroscopy, SEM, XRD, EDAX, FTIR, and AAS. Toxicity tests were observed at 96 hours in different concentrations of magnesium oxide nanoparticles exposed in mrigal. The sub-lethal studies were conducted for 14 days. Biochemical and haematological parameters were estimated. The UV-Visible absorption spectra demonstrate that magnesium oxide nanoparticles were measured in the wavelength range of 200-1100. Morphological characteristics were observed by using SEM at the wavelength of 2µm. EDAX spectrum showed two peaks located between 0KeV to 10KeV. The peak at 1.2KeV comes from magnesium and the second peak at 0.3KeV indicates oxygen. XRD results confirmed the crystalline size of magnesium oxide nanoparticles and the approximate particle size is 10nm. The FT-IR spectrum was analysed at the wavenumber range of 400-4000cm-1and the functional groups are alcohols, alkaline, anhydride and sulfonate. Atomic absorption spectroscopies estimate the convergence of metallic components in magnesium oxide. Total protein and lipid content in the muscle, gill and liver of Mrigal is significantly increased and carbohydrate content decreased when reared in different magnesium oxide concentrations. All the blood parameters are gradually increased from lower concentration to higher concentration. In the DPPH assay, when hydrogen donating ability with the DPPH, the red colour turns to yellow colour. Hydroxyl radical scavenging assay showed that the polyunsaturated fatty acid moieties of cell damage in lower concentration compared to higher concentration.