ASSOCIATION OF LIPOPROTEIN LIPASE GENE ASN291SER DNA POLYMORPHISM WITH PLASMA LIPID LEVELS AND BLOOD PRESSURE LEVELS IN HEALTHY POPULATION OF SERBIA

Abstract

Summary: The lipoprotein lipase (LPL) Asn291Ser polymorphism has been determined in 313 healthy subjects from Serbia. LPL variants were detected by mismatch PCR followed by RsaI restriction digestion and 5% polyacrylamide gel electrophoresis. The relationship between LPL Asn291Ser polymorphism and plasma lipid levels, and association between Asn291Ser polymorphism and arterial blood pressure were found. Significant decrease in high-density lipoprotein cholesterol levels (p<0.05) and significantly higher values of systolic blood pressure (SBP) (p<0.01) were found in females carrying the Ser291 allele. Male carriers of Ser291 allele had significantly lower levels of SBP (p<0.01). Findings from our study could be of importance for further research of pathological state regarding lipid metabolism and other risk factors of vascular diseases in Serbian population.