ACUTE PHASE OF MYOCARDIAL INFARCTION: EFFECT OF If-CURRENT BLOCKADE ON ISOLATED RAT HEART

A. M. Kuptsova, R. K. Bugrov, N. Ziyatdinova, T. L. Zefirov
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Abstract

If-current density in cardiomyocytes changes not only with age, but also with pathophysiological hypertrophy. Thus, studies of pathological conditions in experimental animal models allow us to study If-current role in pathophysiological processes occurring in the heart after myocardial infarction (MI). The objective of the study is to examine the effect of If-currents blockade on the isolated heart of rats with modelled acute myocardial infarction (AMI) and sham-operated (SO) rats. Materials and Methods. MI was reproduced by the left coronary artery ligation. The study was conducted on a Langendorff-isolated heart (ADInstruments). During AMI, the authors studied chronotropy, inotropy and its temporal characteristics, and blood supply of the rat heart. Results. It was found that the initial values of contractile force and coronary flow were significantly lower in the group with an experimental AMI model. There were no differences in heart rate and temporal characteristics of contractile force. If-current blockade (ZD7288, 10-9 M, 10-5 M) reduced the contractile force and coronary flow of the isolated heart in both experimental groups, the changes were more pronounced in the SO group. If-current blockade (10-9 M) in the SO group led to tachycardia, and in the AMI group - to bradycardia. ZD7288 (10-5 M) reduced heart rate in both groups, but in the AMI group, bradycardia was more pronounced. It is possible that the obtained dynamics is associated with a change in the if-current density in the dyscrasic myocardium. Thus, it is obvious that If-currents are not only involved in the regulation of various MI heart functions, but their role changes at different stages of experimental myocardial infarction.
心肌梗死急性期:If-CURRENT阻断对离体大鼠心脏的影响
心肌细胞内if电流密度不仅随年龄的增长而变化,而且随病理生理肥厚而变化。因此,在实验动物模型中对病理条件的研究使我们能够研究If-current在心肌梗死(MI)后发生的心脏病理生理过程中的作用。本研究的目的是研究if电流阻断对模拟急性心肌梗死(AMI)大鼠和假手术(SO)大鼠离体心脏的影响。材料与方法。左冠状动脉结扎术再现心肌梗死。该研究是在langendorff离体心脏(ADInstruments)上进行的。急性心肌梗死(AMI)时,观察大鼠心肌变时性、收缩性及其时间特征,以及心肌血供情况。结果。实验发现AMI模型组心肌收缩力和冠状动脉血流初始值明显降低。在心率和收缩力的时间特征上没有差异。如果电流阻断(ZD7288、10-9 M、10-5 M)使离体心脏收缩力和冠状动脉血流减少,则以SO组明显。if -电流阻断(10-9 M)在SO组导致心动过速,在AMI组-心动过缓。ZD7288 (10-5 M)降低两组心率,但AMI组心动过缓更为明显。所获得的动力学可能与异常心肌中if电流密度的变化有关。由此可见,if -电流不仅参与各种心肌梗死心功能的调节,而且在实验性心肌梗死的不同阶段,其作用发生了变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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