Synthesis of Pyrazole Derivative Incorporating Fluorine in the Aromatic Substitution for Physiological and Pharmacological Studies

Abstract

Over the past decade, drug resistance has become a growing problem in the treatment of infectious diseases caused by bacteria, fungi and viruses. In particular, resistance of bacterial pathogens to current antibiotics has emerged as a measure of health problems. This is especially true in the case of infectious diseases such as pneumonia, meningitis and tuberculosis, which would once have been easily treated with antibiotics, is no longer so readily treated. At present, all widely used antibiotic, including some of the agent such as streptogramins and new generation fluoroquinolones are subjected to bacterial resistance. The search for new antimicrobial agent is one of the most challenging tasks to the medicinal chemist. A search for new antimicrobial compounds with potent and minor toxicity continues to be a region of exploration in medicinal chemistry. It has been well recognized that nitrogen containing heterocyclic molecule is a seat of diverse medicinal activities. Heterocyclic nucleus Pyrazole constitutes an important class of compounds for new drug development. The synthesis of these derivatives and investigation of their chemical and biological behavior have gained more importance in recent decades. Owing to the pharmacological importance of Pyrazole, and its derivatives, in the present work, it has been contemplated to combine various biologically active moieties with these heterocycles through active functional systems to form new molecular framework. Prompted by the therapeutic importance of these heterocyclic derivatives, in the present research work we have synthesized Pyrazole with fluorinated aromatic rings, and its derivatives through multistep reaction. Appropriate synthetic methodologies have been developed for the synthesis of new molecules. Their purification techniques have been established. All the newly synthesized compounds have been characterized by 1H NMR, 13C NMR, mass spectral, IR and elemental analyses. Also structures of a few molecules have been confirmed by X-ray crystallographic analysis. Further, the target molecules have been evaluated for their in vitro antibacterial and antifungal activity. From the pharmacological evaluation, it has been observed that some of Pyrazole, derivatives showed good antimicrobial activity