Studies on polymer capped quantum dots and peptide self-assembly using fluorescence coupled capillary electrophoresis

Abstract

A new bioprobe was designed to study the quantum dots (QDs) based bioanalysis. Then, fluorescence coupled capillary electrophoresis (CE-FL) was utilized to probe the self-assembly of a bifunctional polymer (PAA-g-MEA) capped QDs and ATTO labeled peptide (ATTO-EA5H6). Fluorescence resonance energy transfer (FRET) was detected readily by CE-FL. The FRET signal increased with ATTO-EA5H6/QD molar ratio, which plateaued at 4:1.