BODIPY-based Fluorescent Indicators for Lipid Droplets

IF 3.4 Q2 CHEMISTRY, ANALYTICAL
Dr. Jianping Zhu, Nian Kee Tan, Kai Kikuchi, Dr. Amandeep Kaur, Prof. Elizabeth J. New
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引用次数: 0

Abstract

Lipid droplets are essential for cellular lipid storage, playing critical roles in cellular lipid metabolism. Although lipid droplets have drawn intense research in recent years, much remains to be uncovered about the roles of this organelle in biology. Lipid droplet indicators exhibiting large Stokes shifts and improved brightness are therefore in demand. We report two asymmetric BODIPY derivatives, BoL1 and BoL2 bearing a benzothiazole group at the 6-position, which led to bathochromic shifts of 62 nm for BoL1 and 37 nm for BoL2 in fluorescence emission when compared to the reference molecules without the benzothiazole group. The incorporation of the benzothiazole moiety also resulted in a large Stokes shift of 40 nm. BoL1 and BoL2 have been demonstrated to operate as lipid droplet indicators in both confocal and STED imaging. BoL2 showed particularly good cellular retention and was further applied to explore the impact of cellular starvation on the trafficking of lipid droplets.

Abstract Image

基于 BODIPY 的脂滴荧光指示剂
脂滴是细胞贮存脂质的重要细胞器,在细胞脂质代谢中发挥着关键作用。尽管近年来对脂滴进行了深入研究,但关于这一细胞器在生物学中的作用仍有许多问题有待揭示。因此,需要能显示较大斯托克斯偏移和更高亮度的脂滴指示剂。我们报告了两种不对称 BODIPY 衍生物 BoL1 和 BoL2,它们在 6 位上带有苯并噻唑基团,与不带苯并噻唑基团的参考分子相比,BoL1 和 BoL2 的荧光发射分别发生了 62 nm 和 37 nm 的浴色偏移。苯并噻唑基团的加入也导致了 40 nm 的巨大斯托克斯位移。在共聚焦和 STED 成像中,BoL1 和 BoL2 被证明可用作脂滴指示剂。BoL2 显示出特别好的细胞保留性,并被进一步用于探索细胞饥饿对脂滴迁移的影响。
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来源期刊
CiteScore
2.60
自引率
0.00%
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