Dynamic Thiol-Disulfide Homeostasis in Children with Newly Diagnosed Iron Deficiency Anemia

Burçak Kurucu, A. Fettah, Ş. Yeşil, Esra Fırat Oğuz, Ö. Erel, G. Şahin
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Abstract

Objective: Iron is an element, which is found in the structure of antioxidant enzymes and has an important role in the inactivation of reactive oxygen species. Disruption of oxidant-antioxidant balance may be playing a role in the pathogenesis of iron deficiency anemia (IDA). Dynamic thiol-disulfide homeostasis (DTDH) and serum ischemia-modified albumin (IMA) levels are important indicators of pro-oxidant/antioxidant status. In this study, we aimed to evaluate DTDH parameters and serum IMA levels in children with newly diagnosed IDA, who did not receive iron therapy. Material and Methods: Fifty patients diagnosed with IDA and 33 healthy age- and sex-matched control patients were included in the study. DTDH parameters and IMA levels of the patients and control groups were measured. The same parameters were also compared in patients with Hb<7 g/dl (profound IDA) (n:14/50, 28%) and Hb≥7 g/dl (mild-moderate IDA) (n: 36/50, 72%) in the IDA group. The relationship between DTDH parameters in these groups were investigated. Results: Native thiol, total thiol, native thiol/total thiol levels, constituting antioxidant capacity indicators, were found to be significantly lower in IDA patients; while oxidant disulfide, disulfide/native thiol, disulfide/total thiol, and IMA levels were found to be statistically higher compared to those in the control group (p<0.050). When DTDH parameters and IMA levels were examined; there was a positive correlation between antioxidant parameters and a negative correlation between oxidative parameters with hemoglobin and ferritin levels (p<0.050). Also, oxidative parameters were found to be much higher in profound IDA group than in the group with Hb>7 g/dl (p<0.050). Conclusion: In this study, increase in serum disulfide and IMA levels with the decrease in serum native thiol and total thiol levels indicated oxidative stress in IDA patients before treatment, compared to the control group. Evaluation of these indicators in children is important in predicting the toxicity due to IDA.
新诊断缺铁性贫血儿童动态硫-二硫体内平衡
目的:铁是一种存在于抗氧化酶结构中的元素,对活性氧的失活具有重要作用。氧化-抗氧化平衡的破坏可能在缺铁性贫血(IDA)的发病机制中起作用。动态巯基二硫稳态(DTDH)和血清缺血修饰白蛋白(IMA)水平是促氧化/抗氧化状态的重要指标。在这项研究中,我们旨在评估未接受铁治疗的新诊断IDA儿童的ddhh参数和血清IMA水平。材料和方法:50例诊断为IDA的患者和33例年龄和性别匹配的健康对照患者纳入研究。测定患者和对照组ddhh参数和IMA水平。同样的参数也比较了Hb7 g/dl患者(p<0.050)。结论:在本研究中,与对照组相比,治疗前IDA患者血清二硫化物和IMA水平升高,血清天然硫醇和总硫醇水平降低,表明氧化应激。对儿童的这些指标进行评估对于预测IDA的毒性非常重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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