Proposal for a Filterless Fluorescence Sensor for SNP Genotyping

K. Yamasaki, H. Nakazawa, N. Misawa, M. Ishida, K. Sawada
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引用次数: 1

Abstract

This study describes a biosensor for single nucleotide polymorphism (SNP) genotyping based on the filterless fluorescence detection methods. The filterless fluorescence sensor is able to distinguish lights with more than two different wavelengths without optical filters, mirrors, and gratings. From the final results, we observed that emission lights form the “fluorescein isothiocyanate (i.e., FITC)” and the “sulforhodamine 101 acid chloride (i.e., Texas Red)”, which are kinds of fluorescent dyes commonly used in SNP genotyping, were detected with less interference using the filterless fluorescence sensor. Thus, our approach is effective for SNP genotyping with low cost and high portability.
一种用于SNP基因分型的无滤波器荧光传感器的提出
本研究描述了一种基于无过滤荧光检测方法的单核苷酸多态性(SNP)基因分型生物传感器。无滤光片荧光传感器能够在没有光学滤光片、反射镜和光栅的情况下区分两种以上不同波长的光。从最终结果来看,我们发现使用无滤光片荧光传感器可以检测到SNP基因分型中常用的荧光染料“异硫氰酸荧光素(即FITC)”和“硫胺101酸氯化物(即德克萨斯红)”发出的荧光,干扰较小。因此,我们的方法是有效的SNP基因分型,成本低,便携性高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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