Analysis of Whole Blood Quality: Number of Erythrocytes, Leukocytes, Platelets, and pH Value during 28-day Storage

Abstract

: Whole blood contains all the elements of blood such as all blood cells, plasma, and clotting factors. It is used in the treatment of massive bleeding. However whole blood has an expiration time limit. The quality of blood decreases gradually due to storage time and causes blood cell lysis, so it directly affects blood cell counts and pH. The aim of this study was to determine the quality of whole blood during 28day storage. Whole Blood from blood bags containing CPDA-1 was used as a sample. The number of erythrocytes, leukocytes, and platelets was measured by Hematology Analyzer. The results of the study showed that there was a decrease in the number of erythrocytes from 5,02 x 10 6 cell/µL into 4,92x10 6 cell/µL. The number of leukocytes decreased from 6,31x103cell/µL to 3,17 x 103cell/µL. Platelet count also decreased from 195x10 3 cell /µL to 81x10 3 cell/µL. The pH value decreased from 7.2 on to 6.9. This study concluded that there was a decrease in the number of erythrocytes, leukocytes, platelets, and pH. The number of erythrocytes and pH was still normal, while the number of leukocytes and platelets was below the normal range.