PHENOTYPIC DIVERSITY OF BACTERIA OF THE PSEUDOMONAS COMPLEX, ISOLATED FROM PARASALMO MYKISS Walb., ACCORDING TO THE FACTORS OF INVASIVENESS

A. Savushkin, N. Sidorova
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Abstract

The article presents the results of studies of the phenotypic properties of bacteria of the pseudomonas complex group isolated from the microflora of 57 individuals of rainbow trout Parasalmo mykiss Walbaum, 1792, aged 1+ and 2+, selected for a planned study from reservoirs of fishery importance located in Karelia. The emphasis on the phenotypic properties of isolated microorganisms is associated with the increasing epizootic role of virulent strains of Pseudomonas aureofaciens, Ps. chlororaphis, Ps. fluorescens, Ps. putida and others in the development of infectious diseases of salmon fish species. Isolation, identification and study of phenotypic features of isolated pseudomonas cultures were performed according to the recommendations for laboratory diagnostics of Pseudomonas for fish. In parallel, to analyze the degree of invasiveness, the hemolytic activity (HA), as well as the ability to biofilm formation (BPO) and the antilysozyme activity (ALA) of bacteria were evaluated. It was found that the isolated phenotypic variants of pseudomonas bacteria have a pronounced invasiveness, which is more manifested in the form of hemolytic and anti-lysozyme activity.
假单胞菌复合体细菌的表型多样性。,根据侵入性因素
本文介绍了从1792年1岁和2岁的虹鳟鱼Parasalmo mykiss Walbaum的微生物群中分离出的假单胞菌复合群细菌的表型特性的研究结果,这些虹鳟鱼被选为卡累利阿渔业重要水库的计划研究对象。对分离微生物表型特性的重视与金黄色假单胞菌、绿单胞菌、荧光单胞菌、腐臭单胞菌和其他毒力菌株在鲑鱼传染病的发展中日益增加的动物流行病作用有关。根据对鱼类假单胞菌实验室诊断的建议,对分离的假单胞菌培养物进行分离、鉴定和表型特征研究。同时,对细菌的溶血活性(HA)、生物膜形成能力(BPO)和抗溶菌酶活性(ALA)进行评价,分析其侵入程度。发现分离的假单胞菌表型变异具有明显的侵袭性,更多表现为溶血和抗溶菌酶活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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