Optical sectioning and fast optical focussing in microscopy

T. Wilson
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Abstract

The fundamental property, which any optical microscope that is to be used to finally produce three-dimensional images of a volume specimen must possess, is the ability to image efficiently (and infocus) only those regions the specimen that lie within a thin section in the focal region of the microscope. In order to image a three-dimensional volume of a thick specimen it is necessary to take a whole series of such thin optical sections as the specimen is moved axially through the focal region. There are many methods to produce optical sectioning of which the confocal optical system is just one. We shall review these methods and describe a particularly convenient method of implementation that uses white light illumination and real-time image formation and can lead, amongst other things, to enhanced optical sectioning.
显微镜中的光学切片和快速光学聚焦
任何用于最终产生体积标本三维图像的光学显微镜都必须具备的基本特性是能够有效地成像(和聚焦)位于显微镜聚焦区域的薄片内的标本的那些区域。为了成像一个厚标本的三维体积,有必要采取整个系列的这样的薄光学切片的标本轴向移动通过焦点区域。产生光学切片的方法有很多,共聚焦光学系统只是其中一种。我们将回顾这些方法,并描述一种特别方便的实现方法,该方法使用白光照明和实时图像形成,并且可以在其他方面导致增强光学切片。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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