Aktivitas Antelmintik Subfraksi dariFraksi Etanol Daun Pugun Tanoh [Picria fel-teraae (Lour.)]

Popi Patilaya, Dadang Irfan Husori, Henny Sri Wahyuni
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引用次数: 0

Abstract

Pugun tanoh [Picria fel-terrae (Lour.)] merupakan salah satu tumbuhan obat Indonesia yang memiliki potensi sebagai antelmintik. Ekstrak etanol daun pugun tanoh dan fraksi-fraksinya mampu membunuh cacing parasit Ascaris lumbricoides dan Ascaridia gali. Pengujian terhadap aktivitas antelmintik subfraksi dari ekstrak etanol tersebut perlu dilakukan sebagai upaya untuk memperoleh senyawa bioaktifnya. Penelitian ini bertujuan untuk mengetahui aktivitas antelmintik subfraksi dari fraksi etanol daun pugun tanoh. Penelitian dilakukan dengan memfraksinasi 20g fraksi etanol daun pugun tanoh secara kromatografi cair vakum dengan fase gerak landaian n-heksana-etil asetat dan etil asetat-metanol menggunakan fase diam silika gel 60H. Setiap 250 ml cuplikan ditampung dan ditentukan pola kromatogramnya dengan kromatografi lapis tipis menggunakan fase gerak n-heksana-etilasetat (70:30) dan fase diam silika gel GF254. Cuplikan dengan pola kromatografi yang sama dikumpulkan sebagai satu subfraksi. Setelah diuapkan, subfraksi diuji aktivitas antelmintiknya terhadap Pheretima posthuma.Hasil penelitian menunjukkan bahwa fraksi etanol daun pugun tanoh menghasilkan 4 subfraksi yaitu SF1 (0,10%), SF2 (4,00%), SF3 (4,05%), dan SF4 (73,55%). Waktu kematian P. posthuma akibat paparan SF1, SF2, SF3, dan SF4 masing-masing adalah 77,00 ± 1,00 menit; 56,33 ± 1,76 menit; 79,33 ± 1,33 menit; dan 112,33 ± 0,67 menit. Subfraksi dari fraksi etanol daun pugun tanoh memiliki aktivitas antelmintik dimana SF2 lebih kuat dibandingkan SF3, SF1, dan SF4.   Pugun tanoh [Picria fel-terrae (Lour.)] is one of the Indonesian medicinal plants which has the potential as an anthelmintic. Ethanol extract of pugun tanoh leaves and their fractions were able to destroy the parasitic worms Ascaris lumbricoides and Ascaridia. The evaluation of antelmintic activity of subfraction of ethanol extract needs to be done in an effort to obtain its bioactive compounds. This study aimed to determine the anthelmintic activity of subfraction of  pugun tanoh leavesethanol fraction. The study was carried out by fractionating 20g ethanol fraction of pugun tanoh leaves by vacuum liquid chromatography with a mobile phase of n-hexane-ethyl acetate and ethyl acetate-methanol using the stationary phase of silica gel 60H.Each 250 ml aliquot was collected and the chromatogram pattern was determined by thin layer chromatography using the n-hexane-ethylacetate(70:30)  asmobile phase and silica gel GF254 as stationary phase. The Aliquots with same chromatographic pattern were collected as one subfraction. After being evaporated, the subfraction was tested for its anthelmintic activity against Pheretima posthuma. The results showed that ethanol fraction of pugun tanoh leaves produced 4 subfractions namely SF1 (0.10%), SF2 (4.00%), SF3 (4.05%), and SF4 (73.55%). The time of P. posthuma's death due to exposure to SF1, SF2, SF3, and SF4 was 77.00 ± 1.00; 56.33 ± 1.76; 79.33 ± 1.33; and 112.33 ± 0.67 minutes, respectively. Subfraction of ethanol fraction of pugun tanoh leaves has anthelmintic activity in which SF2 was stronger than SF3, SF1, and SF4
芦丁活性以乙醇为成分。
Pugun tanoh [piria felterrae,意为Lour]是一种具有麻醉品潜力的印尼草药。从乙醇提取物和它的衍生物中分离出来,可以杀死蛔虫、蛔虫和蛔虫。对乙醇提取物的衍生物活性进行测试是必要的,以获得其生物活性化合物。本研究的目的是确定普岗天诺叶醇乙醇的衍生物活性。这项研究采用的是一种乙基- 20g的甲基-坦诺叶基苯丙胺衍生物,其对乙醇的基苯丙醇进行基苯丙醇的基苯丙醇平衡性研究。每250毫升的色谱仪是用薄层色谱仪排列和测定的,它们使用n- hektat etilasetat(70:30)和闭环硅胶GF254。用同样的色谱模式收集的快照作为一个子分数。注射后,衍射在死后对他的恒星活动进行了测试。研究结果表明,从乙醇的叶子pugun tanoh中产生四种成分,即SF1(0.10%)、SF2(400%)、SF3(405%)和SF4(73.55%)。暴露造成的死亡时间P . posthuma SF1, SF2 SF3, SF4每人是77.00±1分钟;56.33±1,76分钟;79.33±1,33分钟;和112.33±0,67分钟。pugun tanoh叶乙醇的衍生物具有先行活动,其中SF2比SF3、SF1和SF4更强。Pugun tanoh [piria felterrae]是印尼中部最具潜力的植物之一。乙醇extract of pugun tanoh叶和它们的框架有可能消灭寄生虫虫Ascaris lumbricoides和寄生虫。对乙醇的潜意识活动的评估需要以一种努力来揭露它的生物活性化合物。这个研究可以确定pugun tanoh leavesethanol的潜意识行为。《研究》以20克的排版为基础,由n-hexane-ethyl acetate和ethyl -methanol的移动相位摄影采用二氧化硅凝胶60H。每250英里的阿里卡被收集,其中的chromatogram是通过n-hexane-ethylacetate(70:30)的asmobile phase和cyca gel GF254作为stationary phase而确定的。具有相同chromato图形的alique被收集为一个子帧。在被消灭后,潜意识受到对死后遗传缺陷的影响。结果表明,pugun tanoh的乙醇成分导致了4个subhanol SF1(0.10%)、SF2(4.00%)、SF3(4.05%)和SF4(73.55%)。《文物》(P . posthuma死亡帐款曝光去参加SF1, SF2, SF3和SF4是77点±1点;56。33±1 76;79 33±1。33;和112,respectively 33±0。67分钟。puf2比SF3、SF1和SF4更强
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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