Expression of Neuron Specific Neuronal Nuclei Protein (NeuNNP) by AAV2 Shows Diagnosis Ability in Spinal Cord Injury Using Neural Stem Cells

Abstract

Fibroblast Growth Factor (FGF) shows promising therapy for Spinal Cord Injury (SCI). Neural stem transplantation has been on the role implementing tactics to repair SCI. Considering the hostile hypoxia condition in SCI, Adeno Associated Virus 2 (AAV2) a prototype to AAV is being used for most trials. Basic FGF gene was used in that hostile environment, results showed improved functional recovery in SCI. The regulation of this gene FGF is transduce to yield AAV2-5HRE-bFGF. The improved scores on inclined plane test of BBB (Basso-BettieBresnahan) scale and footprint analysis of functional recovery when compared with vehicle control of AAV2 treatment showed inclination in NeuNNP, neuromodelin GAP43 and neurofilament NF200 and declination in Glial Fibrillary Acidic Protein (GFAP). Other expressions such as LC3-II and Beclin 1 and autophagy-associated proteins all show decline,