Characterization of Permittivity and Conductivity for ESKAPE Pathogens Detection

Abstract

This paper is to addresses the important diagnostic / detection technology gap by describing a rapid, portable, low-cost, and easy-to-use microfluidic, Dielectrophoresis Lab: On-A-Chip based system for detecting the Enterococcus faecium, Staphylococcusaureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter (ESKAPE) bacterial pathogens that are most commonly associated with antibiotic resistance. However, in this study was focus used the sizes of ESKAPE pathogens to identified the unique identification by translated it into dielectric properties. The MATLAB software was used to analyze and simulated the dielectric properties of each ESKAPE species based on their sizes. The MATLAB simulation was successfully conducted to identified the permittivity and conductivity crossover frequencies for all of Enterococcus faecium, Staphylococcusaureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter species were 1.07 MHz, 0.91 MHz, 1.17 MHz, 0.62 MHz, 0.69 MHz and 0.76 Mhz with each average radius 0.59 µm, 0.687 µm, 0.545 µm, 0.962 µm, 0.875 µm and 0.81 µm respectively. The smallest size of ESKAPE species Klebsiella pneumonia, r of 0.545 µm have the higher crossover frequency (COF) of 1.17 MHz. In contrast the Acinetobacter baumannii species have the largest size, r of 0.962µm but it has lower COF of 0.62MHz.