PHARMACOLOGICAL ACTIVITY OF NEW COMPOSITION WITH A DIMETHYLACETAMIDE DERIVATIVE IN INFRAORBITAL ANESTHESIA IN RATS WITH PERIODONTITIS

Abstract

The safety and local anesthetic activity of the pharmaceutical composition LHT-15-13 containing a dimethylacetamide derivative, an antioxidant and a vasoconstrictor in rats with chronic periodontitis was studied in the work. White laboratory mice were used to determine acute toxicity with intravenous and subcutaneous administration of a 2% aqueous solution of LHT-15-32. In rats with experimental periodontitis, the threshold of pain sensitivity of the second upper molar was determined. The activity of oxidative stress and total antioxidant activity was determined in the mucous membrane of the gums of rats by the method of induced chemiluminescence. Local changes in periodontal tissues were assessed by quantitative enzyme immunoassay. The acute toxicity of the pharmaceutical composition when administered intravenously and subcutaneously was lower than that of the dimethylacetamide derivative with succinic acid LHT-600. LHT-15-13, when administered infraorbitally, caused deep anesthesia that lasted more than 70 minutes, activated the local antioxidant defense system and reduced the level of IL-1β in the gum tissue, caused tissue repair around the impacted upper molar in rats five days after injection. Thus, the pharmaceutical composition can be considered as an effective and safe agent for pain relief of the upper molars in periodontitis.