Identification of a Gordonia sp. strain producing carotenoids

Y.Z. Wang, J.H. Wang, Z. Liu, J.F. Zhao, S. Zhou
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引用次数: 2

Abstract

A pigment-producing strain HBUT-Y was indentified by morphological characterization, physiological & biochemical taxonomy and molecular phylogeny, the carotenoids components in the strain were analyzed. It was found that similarity between the HBUT-Y and strains in genus Gordonia ranged from 95% to 99% through 16S rRNA sequence comparison. Substrate utilization data were provided by Biolog automated microbial identification system. combining with the result, HBUT-Y was preliminary indentified as a Gordonia sputi. Major pigment components in acetone extraction of HBUT-Y were assayed as carotenoids in detection of optical absorption in 452nm,477nm,507nm.
产类胡萝卜素的戈登属菌株的鉴定
通过形态鉴定、生理生化分类和分子系统发育鉴定了一株产色素菌株HBUT-Y,并对其类胡萝卜素成分进行了分析。通过16S rRNA序列比较,发现HBUT-Y与Gordonia属菌株的相似性在95% ~ 99%之间。底物利用数据由Biolog自动微生物鉴定系统提供。结合实验结果,初步鉴定HBUT-Y为高氏菌。在452nm、477nm、507nm光吸收检测中,以丙酮提取液中主要色素成分为类胡萝卜素。
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