Urine Cytomorphological Changes Associated with Diabetes Millets Type II

Abstract

Patient with diabetes millets are at high risk for urinary tract infection. The diabetic condition contributes for initial and progression of urinary tract complications, many studies have shown that a variety of diabetes mellitus related to abnormalities in the urinary tract including severe urinary tract infections. This study was designed to assess the changes in the urinary tract in a patient with diabetes millets. 40 mid stream urine samples were collected from patients with diabetes mellitus type II and 40 midstream urine samples were collected from healthy participants as a control group. First, the slides were treated with egg white as adhesive media then the samples were centrifuged at 2000RPM for 10 min at room temperature. The supernatant was discharged and the deposit put on 20mm around the slide, before the smear was dried it has been fixed immediately by 98% ethanol alcohol. The fixed dried smear are hydrated in 95% alcohol for 2 min, through 70% alcohol for 2 min, rinse in water for 1 min, stained in harries hematoxylin for 5 min, rinsed in water for 2 min, differentiated in 0.5% aqueous hydrochloric acid for 10 seconds, rinsed in water for 2 min, blued in Scott‘s tap water substitute for 2 min, rinsed in water for 2 min, dehydrated in 70% alcohol for 2 min, dehydrated in 95% alcohol for 2 min, dehydrated in 95% alcohol for 2 min, stained in OG6 for 2 min, rinsed in 2 changed 95% alcohol for 2 min in each, stained in EA50 for 3 min, dehydrated in 95% alcohol for 1 min, through absolute alcohol, cleared in xylene and mounted in DPX. This study has shown that individuals with diabetes millets are at higher risk for urinary tract infection. Increased susceptibility in patients with diabetes millets is positively associated with increased duration and severity of diabetes. The number of females in the case group (23) and male is (17). The age in the case were (30-82) and in control were between (21-31), 6 of the males were infected and 9 of the females were infected. The infection was severe progressively with the duration of disease onset. Also, the infections were more frequent in older subjects and we found that the female subjects are more frequently infected than the male subjects. Keywords: Diabetes millets, Cytology, Urine