Catheter-associated urinary tract biofilms: can achyranthes aspera extract work against them?

A. Geddawy, K. Shamna, M. Poyil
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Abstract

Catheter-associated urinary tract infection is mainly related to biofilm on the catheter surface which provides the opportunity for colonization and attachment resulting complex structured biofilm formation makes development of antibiotic resistant organism creates management of CAUTI is more critical. Therefore, there is an alarming call for drug development against multidrug resistant organisms. Hence, our study attempted the antibacterial activity of methanolic extract of Achyranthes aspera against Staphylococcus aureus one of the prevalent organisms involved in CAUTI. The A. aspera antibacterial activity was determined against S. aureus and minimum inhibitory concentration of A. aspera against S. aureus calculated was as 3.6 mg/ml which needed to inhibit the growth of tested microbe. The colony forming ability of S. aureus was studied in the presence of A. aspera methanolic extract using crystal violet staining method and the colony formation was observed after the MIC level indicates colony formation increases when decreasing the concentration of A. aspera. To ascertain the effect of A. aspera on biofilm formation after treatment with various concentrations, the biofilm formation assay performed. The A. aspera can able to reduce the biofilm formation as 80 and 88% for 1X MIC and 2X MIC against S. aureus indicating the antibiofilm forming ability. To prevent the microbial colonization on catheter surface, the coating of catheter with methanolic extract of A. aspera was investigated for its antibacterial activity against S. aureus using in vitro bladder model. The clear zone formations around the catheter piece indicating the anti-adhesive property of A. aspera against S. aureus. Collectively, methanolic extract of A. aspera can pave the way for new antibacterial agent against S. aureus.
导尿管相关的尿道生物膜:牛膝草提取物能对抗它们吗?
导尿管相关性尿路感染主要与导尿管表面的生物膜有关,这为定植和附着提供了机会,因此结构复杂的生物膜的形成使得抗生素耐药生物的发展创造了对CAUTI的管理更加关键。因此,开发针对多重耐药生物的药物的呼声非常高。因此,我们的研究尝试了牛膝甲醇提取物对CAUTI的主要病原菌之一金黄色葡萄球菌的抑菌活性。测定了葡萄球菌对金黄色葡萄球菌的抑菌活性,计算出葡萄球菌对金黄色葡萄球菌的最低抑菌浓度为3.6 mg/ml,达到抑制被试微生物生长的要求。用结晶紫染色法研究了金黄色葡萄球菌在紫荆醇提物作用下的菌落形成能力,在MIC水平显示随着紫荆浓度的降低菌落形成增加后观察菌落形成。为了确定不同浓度处理后曲霉对生物膜形成的影响,进行了生物膜形成试验。1倍MIC和2倍MIC对金黄色葡萄球菌的生物膜形成减少率分别为80%和88%,表明金黄色葡萄球菌具有抗生物膜形成能力。为了防止细菌在导管表面的定植,采用体外膀胱模型研究了曲霉甲醇提取物涂覆导管对金黄色葡萄球菌的抗菌活性。导管片周围形成清晰的区域,表明曲霉对金黄色葡萄球菌具有抗粘附性。综上所述,沙曲霉甲醇提取物可为开发新型金黄色葡萄球菌抗菌药物铺平道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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