Sample Preparation on Micro-Electrode-Dot-Array Digital Microfluidic Biochips

Abstract

Sample preparation in digital microfluidics refers to the generation of droplets with target concentrations for onchip biochemical applications. In recent years, digital microfluidic biochips (DMFBs) have been adopted as a platform for sample preparation. However, there remain one major problem associated with sample preparation on a conventional DMFB. For conventional DMFBs, only a (1:1) mixing/splitting model can be used, leading to an increase in the number of fluidic operations required for sample preparation. To overcome the drawback, we adopt a next generation DMFB platform, referred to as micro-electrode-dot-array (MEDA), for sample preparation. We propose the first sample preparation method that exploits the MEDA-specific advantages of fine-grained control of droplet sizes and real-time droplet sensing. Experimental demonstration using a fabricated MEDA biochip and simulation results highlight the effectiveness of the proposed sample-preparation method.