Orta Karadeniz Bölgesi’nde Pseudomonas savastanoi pv. phaseolicola ve Xanthomonas axonopodis pv. phaseoli’nin izolasyonu ve tanılanması

Abstract

In 2013-2014, surveys were conducted in order to investigate the presence of Pseudomonas savastonoi pv. phaseolicola ( Psp ) halo blight and Xanthomonas axonopodis pv. phaseoli (common bacterial blight) ( Xap ) in common bean production areas of Samsun, Tokat, Amasya at Middle Black Sea region. Total, 154 common bean fields were surveyed and their leaf and bean samples were collected with typically resembling symptoms using guided sampling method. Total of 72 purified bacterial isolates were obtained. All the isolates assayed in pathogenicity, morphological, biochemical tests. Further identification methods were conducted with LOPAT tests and molecular analyses (PCR), their results have revealed that 30 isolates were Psp and 17 isolates were Xap . Psp isolates were separately inoculated on 8 bean varieties to determine their races according to phenotypic reactions. Their reactions on 8 bean varieties were revealed that all Psp isolates were Psp race 1. This work are proved that races of Psp isolates using universal separation set at Middle Black Sea region in which supplies about 16 % of the total bean production in Turkey.