Perylene diimide-based organic fluorescent nanorods for live cell imaging

Meng-Meng Song, Hong Bi, Liang Wang, Le Yu
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引用次数: 2

Abstract

Cell imaging with fluorescent, magnetic or bimodal nanoparticles is currently undergoing a rapid development. The goal of this study was to evaluate the feasibility and efficiency of cell imaging by using a novel kind of organic fluorescent nanorods without any other dye staining or antibody labeling. Perylene-3, 4, 9, 10-tetracarboxdiimide (PDI) nanorods with uniform diameters (ca. 50 nm) and in a length range of 100–600 nm were fabricated by a template-assisted self-assembly method. The PDI nanorods showed a strong fluorescent emission in the visible range and favorable dispersity in aqueous solution. Human hepatoma (HepG2) cells, Hela cells and human embryonic fibroblasts (HEFs) were chosen as models to investigate the cytotoxicity and the cell imaging effect. MTT assays demonstrated the cytotoxicity of PDI nanorods was concentration-dependent and the 72 h IC50 value were in the range of 42–55 μg/mL which is much higher than that of reported quantum dots (QDs). Particularly, con-focal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM) observations indicated PDI nanorods were able to be internalized into the cells very readily and mostly located in the cytoplasm. Moreover, they were found exhibiting a stable, bright fluorescence as long as several hours within the cells. Besides, the uptake of PDI nanorods was found through an energy-dependent, endocytosis-mediated process.
基于苝二亚胺的有机荧光纳米棒用于活细胞成像
荧光、磁性或双峰纳米粒子的细胞成像技术目前正处于快速发展阶段。本研究的目的是评估一种新型有机荧光纳米棒在不使用任何其他染料染色或抗体标记的情况下进行细胞成像的可行性和效率。采用模板辅助自组装法制备了直径均匀(约50 nm)、长度在100 ~ 600 nm范围内的苝- 3,4,9,10 -四羧基二亚胺(PDI)纳米棒。PDI纳米棒在可见光范围内具有较强的荧光发射,在水溶液中具有良好的分散性。以人肝癌(HepG2)细胞、Hela细胞和人胚胎成纤维细胞(HEFs)为模型,研究其细胞毒性和细胞成像效应。MTT实验表明,PDI纳米棒具有浓度依赖性,72 h IC50值在42 ~ 55 μg/mL范围内,远高于已有报道的量子点(QDs)。特别是,共焦激光扫描显微镜(CLSM)和透射电子显微镜(TEM)观察表明,PDI纳米棒能够很容易地内化到细胞中,并且大部分位于细胞质中。此外,发现它们在细胞内表现出稳定,明亮的荧光长达几个小时。此外,PDI纳米棒的摄取是通过能量依赖的、内吞介导的过程被发现的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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