Protective Effect of Quercetin Administration in Bacterialinduced Periodontitis on Rats

JULY ISSUE Pub Date : 2022-07-15 DOI:10.47836/mjmhs.18.4.1
Afianti Sulastri, Indra Mustika S. Pribadi, A. Amaliya, S. Sukrasno, Andreanus A. Soemardji
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Abstract

Introduction: Quercetin is a flavonoid found in a variety of plants, including guava, apples, onions, and tea. It’s been used as an anti-oxidant and anti-inflammatory substance for a long time. This study aims to investigate the effect of quercetin on periodontitis caused by Porphyromonas gingivalis-adhered ligatures. Methods: Eighteen male adult Sprague Dawley rats were divided into 3 groups, namely the control group (C, n=6) and the other two groups that received quercetin at 45mg/kg/day as a preventive (Qp, n=6) and a curative treatment (Qc, n=6), respectively. Under general anaesthesia, periodontitis was induced by placing a 3/0 non-resorbable sterile silk thread around the mandibular incisor teeth of eighteen male adult Sprague Dawley rats. The ligature placement caused severe irritation in the periodontal tissue. The animals were euthanized after 14 days of post-induction treatment, and samples of the mandibular portion were kept in formalin and prepared for histological processing to determine the grade of inflammation (GI). The periodontal pocket depth (PPD) was measured using the Michigan-O probe with Williams marks at the mesial and lingual sites of the rat’s incisors tooth to determine the clinical parameter. Results: Qp showed the best improvement, in both parameters, clinically (PPD score, p=0,0018 at the lingual site, and p=0,0264 at the mesial site) and histologically (GI, p=0,0002). Significant differences were found in preventing clinical attachment-loss statistically (p<0,05) on Qp, better than the Qc at an equal dose (p<0,05). Conclusion: This finding suggests that quercetin administered as a preventive measure (Qp) may promote the healing process of gingiva in periodontitis conditions better than the control group and curative group (Qc).
槲皮素对大鼠细菌性牙周炎的保护作用
简介:槲皮素是一种类黄酮,存在于多种植物中,包括番石榴、苹果、洋葱和茶。长期以来,它一直被用作抗氧化和抗炎物质。本研究旨在探讨槲皮素对牙龈卟啉单胞菌结扎所致牙周炎的治疗作用。方法:雄性成年sd大鼠18只,分为3组,即对照组(C, n=6),另两组分别给予槲皮素45mg/kg/d预防(Qp, n=6)和治疗(Qc, n=6)。在全身麻醉下,用3/0不可吸收的无菌丝线包裹18只成年雄性sd大鼠下颚门牙,诱导牙周炎。结扎的位置对牙周组织造成严重的刺激。诱导治疗14天后将动物安乐死,下颌部分标本保存在福尔马林中,准备进行组织学处理以确定炎症等级(GI)。采用Michigan-O型探针和Williams标记在大鼠门牙内侧和舌部测量牙周袋深度(PPD),确定临床参数。结果:Qp在临床(PPD评分,舌部p= 0.0018,内侧p= 0.0264)和组织学(GI, p= 0.002)两方面均有最佳改善。Qp在预防临床依恋丧失方面差异有统计学意义(p< 0.05),在等剂量下优于Qc (p< 0.05)。结论:槲皮素作为预防措施(Qp)对牙周炎患者牙龈愈合的促进作用优于对照组和治疗组(Qc)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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