Evaluation of inhibitor effect on micelium development on Fusarium oxysporum, y F. solani, using three dosis of epazote epazote (Chenopodium ambrosioides L.)

Abstract

Using natural extract of epazote (Chenopodium ambrosioides L.), has shown to control phytopatogenic fungi (P. Aguilar et al., 2013; y J. Black Solis et al., 2017). One mililiter of ethanolic extract at concentrations of 25%, 50%, 100% diluted on sterile distilled water (V:V) has been used to inhibit micelial developement of Fusarium oxysporum, and Fusarium solani, added to PDA Petri dish under a completely randomized experimental design, a statistical analysis was carried out by means of an ANOVA and comparison test of means with Tukey´s multiple range. After eight days of incubation, the outstanding inhibit effect has been observed with 100% and the average diameter of colonies was 62 mm., has been measured on both fungi species. In contrast, 62 mm., and 61 mm., was observed on F. oxysporum and F. solani respectively, and a statistical effect was observed (p≤0.05). With 25% and 50%, micelial inhibit developement has been measured, but no statistical differences between concentrations has been calculated (p≥0.05) for both fungi. The results suggest that ethanolic extract of epazote could be used to control both phytopathogenic fungi.