The proliferation site of B-lymphocytes and the role of follicular dendritic cell in secondary lymphoid follicle of the tonsil.

H. Orui, M. Yamakawa, Kazuhiko Yamada, T. Ogata, T. Tsunoda, T. Tsuge, K. Niino, Akihiko Suzuki, A. Suda, Yoshihiro Watanabe, F. Yuda, Y. Imai
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Abstract

The proliferation site of B lymphocytes in secondary lymphoid follicle of the tonsil was evaluated. Five follicles were selected to detect histon H3 mRNA as marker of cells in S-phase of the cell cycle by in situ hybridization. Moreover, 11 follicles were selected and stained immunohistochemically with serial frozen sections with cyclin B1 as a G2-M phase marker, p34cdc2 as a marker mainly of G2-M phase, cyclin E as a marker mainly of late G1 phase, and Ki-67 as a marker of cells in the cell cycle. Secondary lymphoid follicles were divided into five zones by the HE stain and the expression of CD23 (Fc e receptor II) to calculate the positive rate of histon H3 mRNA and these proteins. The positive rates of histon H3 mRNA, cyclin B1, and p34cdc2 were the highest in the dark and the outer zones. The result implies that the outer zone as well as the dark zone is a proliferatation site of B cells.Nextly, the phase of the cell cycle of B cells in the follicular dendritic cell (FDC)-lymphocytes clusters, which were obtained from the germinal center of the secondary lymphoid follicle of human tonsils, is evaluated. The rate of cyclin B1-positive lymphocytes in the cluster (4.6%) is lower than that of the lymphocytes out of the cluster in germinal center (27.7%) and, moreover, the rate of Ki-67-positive cells in the cluster (35.9%) is lower than that of cells out of clusters (61.5%). The result shows that few G2-M phase lymphocytes are retained in the FDC-lymphocytes clusters and support that the B lymphocytes actively do not proliferate in the clusters.
扁桃体次级淋巴滤泡中b淋巴细胞的增殖部位及滤泡树突状细胞的作用。
观察扁桃体次淋巴滤泡中B淋巴细胞的增殖部位。选择5个卵泡,原位杂交检测组蛋白H3 mRNA作为细胞周期s期细胞的标志物。选择11个卵泡,用连续冷冻切片进行免疫组织化学染色,细胞周期蛋白B1为G2-M期标志物,p34cdc2主要为G2-M期标志物,细胞周期蛋白E主要为G1期晚期标志物,Ki-67为细胞周期中的细胞标志物。通过HE染色和CD23 (Fc e受体II)的表达将继发性淋巴滤泡分为5个区,计算组蛋白H3 mRNA和这些蛋白的阳性率。组蛋白H3 mRNA、细胞周期蛋白B1和p34cdc2在暗区和外区阳性率最高。结果表明,外区和暗区都是B细胞的增殖部位。接下来,我们评估了从人类扁桃体次级淋巴滤泡生发中心获得的滤泡树突状细胞-淋巴细胞簇中B细胞的细胞周期阶段。细胞周期蛋白b1阳性淋巴细胞比例(4.6%)低于生发中心细胞簇外淋巴细胞比例(27.7%),ki -67阳性细胞比例(35.9%)低于细胞簇外细胞比例(61.5%)。结果表明fdc -淋巴细胞簇中保留少量G2-M期淋巴细胞,支持B淋巴细胞在簇中不主动增殖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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