Anti-inflammatory activities of compounds isolated from Amanita caesarea collected in Lam Dong province

Abstract

Five natural secondary metabolites as cinnamic acid (1), (+)-catechin (2), (-)-epicatechin (3), p-coumaric acid (4), and ferulic acid (5) were isolated from Amanita caesarea based on anti-inflammatory activity-guided extraction. Their structures (1-5) were determined by NMR spectra as well as by comparison with previously reported literature. Compounds 3 and 5 have been isolated from A. caesarea for the first time. The anti-inflammatory activity through inhibition of nitric oxide (NO) production of isolates (1-5) was evaluated. Among them, compounds 2 and 3 exhibited strong inhibitory activity with IC50 values of 4.8 and 5.7 μM, respectively. Compounds 4 and 5 with IC50 values of 18.4 and 9.6 μM, respectively showed moderate inhibitory activity. The results proposed that A. caesarea might exert anti-inflammatory effects due to its mainly NO-inhibitory constituents