Evaluation of the Bioactivity of Some Traditional Medicinal Plants Using the Brine Shrimp Lethality Test

The Kenya Veterinarian Pub Date : 2007-10-29 DOI:10.4314/KENVET.V28I1.39536

D. Gakuya, J. Mbaria, P. Mbithi, R. Munenge

引用次数: 1

Abstract

The purpose of this experiment was to evaluate the bioactivity of extracts of Chrysanthemum cinerariaefolium Vis Albizia antihelmintica A. Brogn, Maerua edulis (Gilg) De Wolf, Maerua subcordata (Gilg & Bened) De Wolf and Myrsine Africana L. which are used traditionally as antihelmintic by using brine shrimp lethality test. Serial dilutions of 1000g/ml, 100g/ml and 10 mg/ml of the extracts were put in five test tubes. Ten (10) brine shrimp larvae were immersed into each of the test tubes and the number surviving after 24 hours counted and the percentage mortality and LC for each extract was determined. C. cinerariaefolium (pyrethrins) was active (LC 50 of 18g/ml. The methanol extracts of Maerua edulis, Maerua subcordata and Myrsine Africana were not active (LC 50 The Kenya Veterinarian Vol. 26 2004: pp. 8-11

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用盐水对虾致死试验评价几种传统药用植物的生物活性

本试验旨在通过盐水对虾致死试验，评价传统杀虫药菊(Chrysanthemum cinerariaefolium Vis Albizia antihelmintica A. Brogn)、毛蕊花(Maerua edulis (Gilg) De Wolf)、毛蕊花(Maerua subcordata (Gilg & Bened) De Wolf)和非洲丝瓜(mysinine Africana L.)提取物的生物活性。将提取液按1000g/ml、100g/ml和10 mg/ml依次稀释于5支试管中。每个试管中浸泡10(10)只盐水对虾幼虫，计数24小时后的存活率，并测定每种提取物的死亡率和LC()。对拟除虫菊酯(C. cinerariaefolium)有活性(LC:()50%(18g/ml);Maerua edulis, Maerua subcordata和myrine Africana的甲醇提取物没有活性(LC 50肯尼亚兽医Vol. 26 2004: pp. 8-11)

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The Kenya Veterinarian

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