Studies on cytochrome b2 synthesis in Saccharomyces cerevisiae.

Abstract

Synthesis of cytochrome b2 together with that of other hemoproteins is induced by oxygen. It is further stimulated by L-lactate. This is true for the enyzme in mitochondrial as well as cytoplasmic cell fractions. Chloramphenicol and erythromycin do not effect cytochrome b2 biosynthesis, whereas cycloheximide prevents it in aerobicallly adapting cells. Mutants lacking cytochrome b2 activity still exhibit the activities of D-lactate dehydrogenase and D-2hydroxyacid dehydrogenase.