Optimization of Conditions for Ginsenoside Re liposomes Production by Response Surface Methodology

Abstract

In this study, we aimed to optimize the formulation of Ginsenoside Re liposomes using response surface methodology. A transmission electron microscope observed the morphology of G-Re liposomes. Additionally, the microstructure and size of G-Re liposomes were determined by a particle size analyzer. The film dispersion method is a simple and efficient method for liposome preparation. The results showed that the optimal preparation conditions for this method were: phosphatidylcholine/Re ratio of 33.68, phosphatidylcholine/cholesterol ratio of 10.19, and rotary evaporation temperature of 48.50 °C. Under the optimal conditions, the particle size and EE of G-Re liposomes were 173.87 ± 4.74 nm and 60.91% ±1.53, respectively. The G-Re liposomes showed a spherical structure with a smooth surface. The liposomes as carriers improved the stability of G-Re.