Pulsed photoacoustic measurements of suspensions: in case study of mitochnodrial NADH and its phantom

International Conference on Advanced Optical Materials and Devices Pub Date : 2008-09-10 DOI:10.1117/12.815279

Zuomin Zhao, J. Hast, R. Myllylä, M. Känsäkoski

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引用次数: 1

Abstract

The laser photoacoustic spectroscopy (LPAS) can be applied analytically to determine the concentration of an analyte in samples and its absorption variation as a function of time. The LPAS has advantages of higher detection sensitivity, pure absorption measurement and much less interference by background scattering in comparison with traditional optical spectroscopy. On the other hand, the apparatus is simpler and more generally useful than that of fluorescent spectroscopy. In this study, we built a photoacoustic setup of a Q-switched Nd:YAG laser excited at the third harmonic wavelength and a wideband piezoelectric transducer clamped to the side of a cuvette. The samples included NADH solutions, mitochondrial suspensions and Intralipid-ink mixtures. The experiment results show that the lowest detectable absorption of the setup is in the order of 10-5 cm-1; the detectable concentration of NADH in a buffer is as low as 1.67 μM. When monitoring mitochondrial suspensions, the minimal concentration that can be detected is lower than 0.1 mg/mL and the absorption variation caused by full reduction of NAD+ to NADH in mitochondria can be detected.

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悬浮液的脉冲光声测量:在线粒体NADH及其幻影的案例研究中

激光光声光谱(LPAS)可以用于分析样品中分析物的浓度及其随时间的吸收变化。与传统光谱学相比，LPAS具有检测灵敏度高、纯吸收测量、背景散射干扰小等优点。另一方面，该仪器比荧光光谱法更简单，用途更广泛。在这项研究中，我们建立了一个以三次谐波波长激发的调q Nd:YAG激光器和光声装置，并将宽带压电换能器夹在试管的侧面。样品包括NADH溶液、线粒体悬浮液和脂内墨水混合物。实验结果表明，该装置的最低可检测吸收为10-5 cm-1;缓冲液中NADH的检测浓度低至1.67 μM。在监测线粒体悬液时，可以检测到的最小浓度低于0.1 mg/mL，可以检测到线粒体内NAD+完全还原为NADH引起的吸收变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Conference on Advanced Optical Materials and Devices

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