Structural and functional analysis of ribosome subunits isolated from mouse liver following a toxic dose of dimethylnitrosamine.

Abstract

Ribosomal subunits, isolated from mouse liver after a single dose of 50 mg/Kg b. w. of DMN, have been tested for their structural and functional properties. CsCl analysis has shown that after DMN treatment no loss of structural proteins occurs in both 40 S and 60 S ribosome subunits. It has been also demonstrated that these subunits are able to synthetize in the poly-U programmed system at low Mg++ concentrations, in the presence of heterologous initiation factors, at the same efficiency as the corresponding controls.