Hodgkin & Reed-Sternberg細胞がHelper/inducer T細胞型の表面形質を示したホジキン病の1例

Abstract

We report a case of Hodgkin's disease showing helper/inducer T cell phenotype on Hodgkin & Reed-Sternberg cells.A 21 years-old Japanese female was admitted for right cervical lymphadenopathy. Laboratory tests including human T lymphocyte virus-I antibody (HTLV-I antibody) were within normal. A biopsy specimen of the cervical lymph node showed Hodgkin's disease, nodular sclerosis (NS).Immunohistologically, Hodgkin & Reed-Sternberg cells were positive for CD30 (Ki-1), CD15 (Leu-M1), CD25 (IL-2R), HLA-DR, transferrin receptor, CD2 (Leu-5b), CD3 (Leu-4), CD4 (Leu-3a), and CD5 (Leu-1), but were negative for B cell associated antigens and myeloid-monocyte associated antigens. The immunohistochemical data indicate helper/inducer T cell phenotype on Hodgkin & Reed-Sternberg cells.Southern blot analysis revealed six rearranged bands using T cell receptor gene probe (TCR Jγ1 probe), when genomic DNA of the biopsied lymph node was digested with EcoRI. Although the TCRγ gene rearrangement bands were not well evaluated, the finding of TCRγ gene rearrangement is unlikely to consider a clonal prolifaration of Hodgkin & Reed-Stenberg cells with T cell phenotype. It would be speculated in this case that rearranged bands for TCRγ may originate from functional T cell clones with transcription of the CD3 molecule and unique TCRγ rearrangements.The patient is free from the disease 2 years after the biopsy.