Effect of Hesl on bone marrow CD34 + cells in acute myeloid leukemia

Clinical Oncology and Cancer Research Pub Date : 2014-11-30 DOI:10.3969/J.ISSN.1000-8179.20141339

C. Tian, Yongsheng Jia, D. Hu, Yizhuo Zhang

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Abstract

Objective: To determine the effect of Hes1 on bone marrow CD34 cells in acute myeloid leukemia (AML). Methods: Bone marrow mononuclear cells were isolated by using Ficoll. Then, the proportion and cell cycle of CD34+ cells were analyzed by using fluorescence-activated cell sorting (FACS). CD34 cells were cultured in vitro for colony-forming cells (CFC). The expression of Hes1 in CD34 cells was evaluated by using real-time polymerase chain reaction. After upregulating the expression of Hes1 in CD34 cells, the cell cycle was analyzed through FACS, and the colony formation of CD34Hes1 cells was analyzed by CFC. Results: The ratio of CD34 cells in the bone marrow was lower in the AML group than in the control group. In addition, more CD34 cells underwent quiescence in the AML group than in the control group. In vitro assay showed that the colony formation of CD34 cells was lower in the AML group than in the control group. The expression of Hes1 was higher in the CD34 cells from the AML patients than that in the CD34 + cells from normal donors. After Hes1 transduction, more CD34 + cells underwent quiescence and showed weak proliferation. Conclusion: The proportion of CD34 cells in the bone marrow was lower in AML patients than in normal donors. A large proportion of CD34+ cells underwent quiescence, which was related to Hes1, in AML patients.

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Hesl对急性髓系白血病患者骨髓CD34 +细胞的影响

目的:探讨Hes1对急性髓性白血病(AML)患者骨髓CD34细胞的影响。方法:采用Ficoll法分离骨髓单个核细胞。然后采用荧光活化细胞分选(FACS)分析CD34+细胞的比例和细胞周期。将CD34细胞体外培养为集落形成细胞(CFC)。实时聚合酶链反应检测Hes1在CD34细胞中的表达。上调CD34细胞中Hes1的表达后，通过FACS分析细胞周期，CFC分析CD34Hes1细胞的集落形成。结果:AML组骨髓中CD34细胞的比例低于对照组。此外，与对照组相比，AML组有更多的CD34细胞处于静止状态。体外实验显示，AML组CD34细胞的集落形成低于对照组。Hes1在AML患者的CD34细胞中的表达高于正常供者的CD34 +细胞。Hes1转导后，更多的CD34 +细胞处于静止状态，增殖能力较弱。结论:AML患者骨髓中CD34细胞的比例低于正常供者。在AML患者中，大量CD34+细胞处于静止状态，这与Hes1有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Clinical Oncology and Cancer Research

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