Wide grain 4, encoding an alpha-tubulin, regulates grain size by affecting cell expansion in rice

IF 6 1区 农林科学 Q1 AGRONOMY
Yi Liu , Lianan Guo , Guoli Qu, Yang Xiang, Xu Zhao, Hua Yuan, Ting Li, Liangzhu Kang, Shiwen Tang, Bin Tu, Bingtian Ma, Yuping Wang, Shigui Li, Weilan Chen, Peng Qin
{"title":"Wide grain 4, encoding an alpha-tubulin, regulates grain size by affecting cell expansion in rice","authors":"Yi Liu ,&nbsp;Lianan Guo ,&nbsp;Guoli Qu,&nbsp;Yang Xiang,&nbsp;Xu Zhao,&nbsp;Hua Yuan,&nbsp;Ting Li,&nbsp;Liangzhu Kang,&nbsp;Shiwen Tang,&nbsp;Bin Tu,&nbsp;Bingtian Ma,&nbsp;Yuping Wang,&nbsp;Shigui Li,&nbsp;Weilan Chen,&nbsp;Peng Qin","doi":"10.1016/j.cj.2023.05.013","DOIUrl":null,"url":null,"abstract":"<div><p>Rice is one of the three most important food crops in the world. Increasing rice yield is an effective way to ensure food security. Grain size is a key factor affecting rice yield; however, the genetic and molecular mechanisms regulating grain size have not been fully investigated. In this study, we identified a rice mutant, <em>wide grain 4-D</em> (<em>wg4-D</em>), that exhibited a significant increase in grain width and a decrease in grain length. Histological analysis demonstrated that <em>WG4</em> affects cell expansion thereby regulating grain size. MutMap-based gene mapping and complementary transgenic experiments revealed that <em>WG4</em> encodes an alpha-tubulin, OsTubA1. A SNP mutation in <em>WG4</em> affected the arrangement of cortical microtubules and caused a wide-grain phenotype. <em>WG4</em> is located in nuclei and cytoplasm and expressed in various tissues. Our results provide insights into the function of tubulin in rice and identifies novel targets the regulation of grain size in crop breeding.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 6","pages":"Pages 1931-1936"},"PeriodicalIF":6.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123000892/pdfft?md5=44d38c2d58780a99e8e0241f440de492&pid=1-s2.0-S2214514123000892-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Journal","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214514123000892","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0

Abstract

Rice is one of the three most important food crops in the world. Increasing rice yield is an effective way to ensure food security. Grain size is a key factor affecting rice yield; however, the genetic and molecular mechanisms regulating grain size have not been fully investigated. In this study, we identified a rice mutant, wide grain 4-D (wg4-D), that exhibited a significant increase in grain width and a decrease in grain length. Histological analysis demonstrated that WG4 affects cell expansion thereby regulating grain size. MutMap-based gene mapping and complementary transgenic experiments revealed that WG4 encodes an alpha-tubulin, OsTubA1. A SNP mutation in WG4 affected the arrangement of cortical microtubules and caused a wide-grain phenotype. WG4 is located in nuclei and cytoplasm and expressed in various tissues. Our results provide insights into the function of tubulin in rice and identifies novel targets the regulation of grain size in crop breeding.

编码α-微管蛋白的 "宽粒4 "通过影响水稻细胞的扩展来调节谷粒大小
水稻是世界三大重要粮食作物之一。提高水稻产量是确保粮食安全的有效途径。谷粒大小是影响水稻产量的一个关键因素;然而,调控谷粒大小的遗传和分子机制尚未得到充分研究。在这项研究中,我们发现了一种水稻突变体--宽粒 4-D(wg4-D),它的谷粒宽度显著增加,而谷粒长度则显著减少。组织学分析表明,WG4 会影响细胞扩展,从而调节谷粒大小。基于 MutMap 的基因图谱和互补转基因实验发现,WG4 编码一种α-微管蛋白 OsTubA1。WG4 的 SNP 突变影响了皮层微管的排列,并导致宽粒表型。WG4 位于细胞核和细胞质中,在多种组织中表达。我们的研究结果揭示了管蛋白在水稻中的功能,并为作物育种中谷粒大小的调控找到了新的靶标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信