Use of a rotary bioreactor for growth and differentiation of mesenchymal stem cells

Abstract

Bone graft generation in vitro is limited by slow nutrient-waste exchange through thick scaffolds and insufficient mechanical stimuli required for induction and differentiation of osteoprogenitor cells, often requiring the use of suitable bioreactors to circumvent these limitations. In this study, we test the suitability of a modified rotary miniPERM™ bioreactor system in inducing proliferation and osteodifferentiation of human foetal mesenchymal stem cell (hfMSC) on poly-ε-caprolactone (PCL)-tricalcium phosphate (TCP) scaffolds.