薄層クロマトグラフィーによるGrayanotoxin I, IIおよびIIIの定量

Abstract

A thin-layer chromatographic method was developed for identifying grayanotoxin (G)-I, G-II and G-III, which are toxic diterpenoids of Leucothoe grayana MAXIM. The optimum onditions for quantitative analysis were as follows: developing solvent, EtOAc: MeOH=37:3; TLC plate, Merck Kieselgel 60 containing 40% H2O; detection agent, 20% phosphotungstic acid in ethanol; charring temperature and time, 90°C and 20 min. Mixtures of G-1, G-II and G-III were analyzed under these conditions, and reliable results were obtained. This method was applied for identifying grayanoid compounds in toxic honey.