Chloroplast Pigments: Structure, Function, Assembly and Characterization

T. H. Brotosudarmo, L. Limantara, R. D. Chandra, Heriyanto
{"title":"Chloroplast Pigments: Structure, Function, Assembly and Characterization","authors":"T. H. Brotosudarmo, L. Limantara, R. D. Chandra, Heriyanto","doi":"10.5772/INTECHOPEN.75672","DOIUrl":null,"url":null,"abstract":"Chlorophyll and carotenoid are vital components that can be found in the intrinsic part of chloroplast. Their functions include light-harvesting, energy transfer, photochemical redox reaction, as well as photoprotection. These pigments are bound non-covalently to protein to make pigment-protein supercomplex. The exact number and stoichiometry of these pigments in higher plants are varied, but their compositions include chlorophyll (Chl) a , Chl b , lutein, neoxanthin, violaxanthin, zeaxanthin and β-carotene. This chapter provides introduction to the structure and photophysical properties of these pigments, how they assemble as pigment-protein complexes and how they do their functions. Various common methods for isolation, separation and identification of chlorophylls and carotenoid are also discussed. methanol and MTBE at the flow rate of 1 mL/min at 30°C. The purified chlorophyll a was directly analyzed to LCMS 8030 (Shimadzu) with an isocratic elution of 0.1% formic acid (FA) in water (10%) and 0.1% FA in methanol (90%) at the flow rate of 0.3 mL/min. MS analysis was operated under the following conditions: (1) heat block temperature = 400°C; (2) desolvation line temperature = 250°C; (3) nebulizing N2 gas flow 3 L/min; (4) drying N2 gas flow 15 L/min; (5) interface 4.5 kV; (6) interface 0.1 ionization","PeriodicalId":281731,"journal":{"name":"Plant Growth and Regulation - Alterations to Sustain Unfavorable Conditions","volume":"51 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"12","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Growth and Regulation - Alterations to Sustain Unfavorable Conditions","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5772/INTECHOPEN.75672","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 12

Abstract

Chlorophyll and carotenoid are vital components that can be found in the intrinsic part of chloroplast. Their functions include light-harvesting, energy transfer, photochemical redox reaction, as well as photoprotection. These pigments are bound non-covalently to protein to make pigment-protein supercomplex. The exact number and stoichiometry of these pigments in higher plants are varied, but their compositions include chlorophyll (Chl) a , Chl b , lutein, neoxanthin, violaxanthin, zeaxanthin and β-carotene. This chapter provides introduction to the structure and photophysical properties of these pigments, how they assemble as pigment-protein complexes and how they do their functions. Various common methods for isolation, separation and identification of chlorophylls and carotenoid are also discussed. methanol and MTBE at the flow rate of 1 mL/min at 30°C. The purified chlorophyll a was directly analyzed to LCMS 8030 (Shimadzu) with an isocratic elution of 0.1% formic acid (FA) in water (10%) and 0.1% FA in methanol (90%) at the flow rate of 0.3 mL/min. MS analysis was operated under the following conditions: (1) heat block temperature = 400°C; (2) desolvation line temperature = 250°C; (3) nebulizing N2 gas flow 3 L/min; (4) drying N2 gas flow 15 L/min; (5) interface 4.5 kV; (6) interface 0.1 ionization
叶绿体色素:结构、功能、组装和表征
叶绿素和类胡萝卜素是叶绿体内在部分的重要成分。它们的功能包括光收集、能量传递、光化学氧化还原反应和光保护。这些色素与蛋白质非共价结合,形成色素-蛋白质超复合物。高等植物中这些色素的确切数量和化学计量是不同的,但它们的组成包括叶绿素(Chl) a、Chl b、叶黄素、新黄质、紫黄质、玉米黄质和β-胡萝卜素。本章介绍了这些色素的结构和光物理性质,它们是如何组装成色素蛋白复合物的,以及它们是如何发挥作用的。还讨论了各种常用的叶绿素和类胡萝卜素的分离、鉴定方法。甲醇和甲基叔丁基醚,流速为1ml /min,温度为30℃。纯化后的叶绿素a以0.1%甲酸(FA)水溶液(10%)和0.1% FA甲醇溶液(90%)等密度洗脱,流速为0.3 mL/min,直接与LCMS 8030 (Shimadzu)进行分析。质谱分析在以下条件下进行:(1)热块温度= 400℃;(2)脱溶线温度= 250℃;(3)雾化N2气体流量3l /min;(4)干燥N2气流量15l /min;(5)接口4.5 kV;(6)界面0.1电离
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信