Analysis of Antibiotic Resistance Genes of the Extensively Drug Resistant Clinical Klebsiella pneumonia Isolates in Vietnam

Abstract

Background: The over using of antibiotics in hospitals, communities and agriculture has raised drug resistance in Vietnam. Objective: The study was to identify the multi antimicrobial resistance gene expression associated with extensively drug resistance phenotype in Klebsiella pneumonia (K.pneumoniae) isolated from clinical samples in the Military Hospital 103 in Vietnam. Material and Method: Seventeen extensively drug resistant (XDR) strains were identified and measured antibiotic susceptibility by using Vitek 2 Compact System. Genes related to the antimicrobial resistant were screened using in-house multiplex PCR at Vietnam Institute of Genome Research. Results: K.pneumoniae showed resistance to almost fifteen antibiotics with very high rates of over 70%, only sensitive to colistin 100%. There is a correlation between carpabenem resistance and XDR status in the K.pnemoniae group. Most of the K.pneumoniae isolates harbored the widely distributed ESBL gene groups. All the isolates carried more than 3