Impregnation of a Bone Marrow Allograft with an Antibiotic Prepared According to the Marburg System, an Original Method

Abstract

The aim: To develop an original method of antibiotic impregnation of bone allograft prepared according to the Marburg bone bank system. Methods. The work was divided into three parts (as part of an experimental study). The first part of the study included the collection and preparation of bone allograft, which was carried out on the basis of the Hospital named after professor Makazhanov, where the femoral heads were harvested, removed during arthroplasty and heat-treated according to the Marburg system of the bone bank. The second stage of the study was to select the optimal antibacterial drug for impregnation, by determining their thermal stability. The determination of thermal stability was carried out at the Department of Microbiology of the Karaganda Medical University. The third stage of the study included conducting experimental work on the development of an impregnation method and evaluating the antibacterial effectiveness of impregnated bone allografts. Results. Heat-treated bone allograft, perforated by the original method using a developed perforation device (the latter received a patent), and then soaked in an antibiotic, showed suppression of the bacterial growth zone by more than 2.5 times (p=0.01), in comparison with other groups. Conclusion. The proposed bone allograft is biodegradable and is close to autologous bone in its physical and mechanical properties. The optimal method of impregnation is bone allograft perforation, developed by an original method using a special perforation device. Keywords: bone allograft, Marburg system, bone bank, antibiotics, osteomyelitis, impregnation, osteo-replacement material.