The Possible Protective Effect of Propolis Against Aluminum Chloride Induced Hepatic Injury in Adult Male Rats. Histological Study

Cytology & Histology Reports Pub Date : 1900-01-01 DOI:10.29011/2688-6421.100013

M. Raafat, G. A. Ibrahim, S. Ezzat

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Abstract

Background: Aluminum is a widely distributed metal in the environment and is extensively used in daily life that provides easy exposure to human. It is known that Aluminum induces toxic effect on the liver. Propolis is a honeybee product with antioxidant properties. Hence, aim of the work was to evaluate the possible protective effect of propolis against hepatic toxicity caused by Aluminum chloride (AlCl3) in rats. Material and methods: The present study was carried out on forty adult male albino rats that were divided randomly into four equal Groups. Group I served as control. Group II: rats were given propolis (50 mg/kg). Group III: received AlCl3 (34 mg/kg). Group IV: received AlCl3 (34 mg/kg) and propolis (50 mg/kg) simultaneously. All medications were given by nasogastric tube for four weeks. Specimens of liver were excised and processed for light and electron microscopic studies. Moreover, biochemical levels of serum liver enzymes were measured. Morphometric and statistical analysis were also done. Results: Administration of propolis alone in Group II had no effect on the hepatic architecture. However, AlCl3 administration in Group III had led elevation of serum liver enzymes and distortion of liver parenchyma. Vacuolated hepatocytes, mononuclear cellular infiltrations and congestion in portal vein branches and blood sinusoids were noticed. Moreover, there was a significant increase in area percentage of collagen fibers and a significant decrease in the optical density of PAS stained glycogen granules as compared to the control Group. By electron microscope, hepatocytes showed many lipid droplets in their cytoplasm, mitochondria with indistinct cristae, and dilated rough endoplasmic reticulum. Hepatic stellate cells associated with collagen fibrils were also detected. However, simultaneous administration of propolis with AlCl3 in Group IV attenuated the biochemical and structural changes induced by AlCl3. Conclusion: Propolis could have a beneficial protective effect against AlCl3 hepatic injury.

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蜂胶对氯化铝致成年雄性大鼠肝损伤的保护作用。组织学研究

背景:铝是一种在环境中分布广泛的金属，在日常生活中被广泛使用，人类容易接触到铝。众所周知，铝对肝脏有毒性作用。蜂胶是一种具有抗氧化特性的蜜蜂产品。因此，本研究旨在探讨蜂胶对氯化铝(AlCl3)致大鼠肝毒性的保护作用。材料与方法:选用成年雄性白化大鼠40只，随机分为4组。第一组为对照组。第二组:给予蜂胶50 mg/kg。III组:给予AlCl3 (34 mg/kg)。IV组:同时给予AlCl3 (34 mg/kg)和蜂胶(50 mg/kg)。所有药物均经鼻胃管给予，持续4周。切除肝脏标本，进行光镜和电镜观察。测定血清肝酶生化水平。并进行了形态计量学和统计学分析。结果:单独给药蜂胶组对肝脏结构无明显影响。而AlCl3给药组血清肝酶升高，肝实质扭曲。肝细胞空泡化，单核细胞浸润，门静脉分支及血窦充血。此外，与对照组相比，PAS染色糖原颗粒的胶原纤维面积百分比显著增加，光密度显著降低。电镜下，肝细胞胞质内脂滴较多，线粒体嵴不清，粗内质网扩张。与胶原原纤维相关的肝星状细胞也被检测到。然而，与AlCl3同时给予蜂胶，在IV组中，AlCl3引起的生化和结构变化减弱。结论:蜂胶对AlCl3肝损伤具有良好的保护作用。

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Cytology & Histology Reports

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