Isolation and Screening of Antagonistic Actinomycetes for Potential Application in The Control of Pathogenic Bacteria in Contaminated Waste

Abstract

Purpose Organic wastes for agricultural use represents a real agronomic interest but also a risk for public and animal health. Actinomycetes present a potential source of bioactive compounds with multiple applications. Methods Among the 88 isolates of actinomycetes obtained from different samples, we selected a strain identified, thanks to macro- and microscopic characters, as Streptomyces sp. SCM21. This strain showed these potentialities to produce antibacterial metabolites against the pathogenic bacteria tested (S. aureus, B. cereus and S. enteritidis) when cultivated in liquid and solid fermentation. Solid-state fermentation was conducted using sewage sludge as substrate. Results This is the first time that sewage sludge has been used to produce antibacterial metabolites by a strain of actinomycetes in solid-state fermentation. In addition, solid-state fermentation moistened with distilled water gave better antibacterial activity with good sporulation of Streptomyces sp. CSM21. Conclusion The product of solid-state fermentation can be used in the management of contaminated waste to control pathogenic bacteria.