A CMOS/Microfluidics Point-of-Care SoC employing Square-Wave Voltcoulometry for Biosensing with Aptamers and CRISPR-Cas12a Enzymes

Abstract

This paper presents a CMOS/microfluidics pointof-care (PoC)SoC for molecular detection using DNA aptamers and CRISPR-associated enzymes (Cas). We take advantage of the signaling property from the electron transfers of the redox reporters and present a square-wave voltcoulometry (SWVC) electrochemical readout circuit to achieve $\gt 100\times$ signal enhancement. The SoC is implemented in 180-nm CMOS technology, integrated with pH and temperature sensors, and consumes a total power of 2.4mW.