CHANGES IN MICROBIOLOGICAL AND PHYSICO-CHEMICAL PROPERTIES DURING COMPOSTING OF SIAM WEED (Chromolaena odorata) AND COWDUNG

A. Akintokun, P. Akintokun, A. O. Obawusi, O. Lawal
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Abstract

Three compost samples were prepared in this study from Siam weed (Chromolaena odorata) and cowdung. Sample A was prepared from Cow dung and siam weed at ratio 100g: 100g, Sample B was prepared from 200g chopped siam weed and sample C contained 200g cowdung. These three sam-ples were composted in plastic drums perforated for aeration and each sample were replicated three times. The content in the drums were regularly turned and monitored at 1, 10, 30 and 60 days for mi-crobiological and physicochemical properties. The microbiological and physicochemical analyses of the compost were carried out using standard procedures. Bacterial, Coliform and Fungal count in-creased from day 1 to the 30th day and thereafter decreased from 30th day to the 60th day in all the composting samples. The bacteria species isolated and identified were Pseudomonas fragilis, Pseu-domonas nitrificans, Proteus mirabilis, E. coli, Streptococcus faecium, Micrococcus luteus, Clostridium perfringes, Bacillus cereus, Proteus morganii, Micrococcus acidophilus. Fungal species were Aspergil-lus flavus, Aspergillus fumigatus, Fusarium oxysporium, Penicillum chrysogenum, Aspergillus niger, Mucor sp. and Saccharomyces cerevisiae. The pH of the composted samples ranges between 5.8 to 6.9. The nitrogen, phosphorus and potassium content increased with days of composting but the heavy metals decreased with days of composting. The sulfatase, phosphatase, dehydrogenase, amyl-ase and cellulose enzymes in the three samples increased from day 1 to the 60th day. Sulfatase en-zyme which was the highest ranged from 25 to 76.5% in the three sample, phosphatase (14 to 60.5%), dehydrogenase (20.5 to 55.0%), cellulose (16.5 to 49%) and amylase which was the least enzyme recorded ranged from 5.0 to 38%.
暹罗草和牛粪堆肥过程中微生物学和理化性质的变化
本研究以暹罗草(Chromolaena odorata)和牛粪为原料制备了3种堆肥样品。样品A由牛粪和暹罗杂草按100g: 100g的比例配制而成,样品B由切碎的暹罗杂草200g配制而成,样品C含有200g牛粪。这三个样品在塑料桶中进行堆肥穿孔曝气,每个样品重复三次。在第1、10、30和60天定期翻转桶内的内容物,监测其微生物和理化性质。采用标准程序对堆肥进行微生物学和理化分析。所有堆肥样品的细菌、大肠菌群和真菌数量在第1天至第30天呈上升趋势,在第30天至第60天呈下降趋势。分离鉴定的细菌种类有脆弱假单胞菌、硝化假单胞菌、奇异变形杆菌、大肠杆菌、粪链球菌、黄体微球菌、荚膜梭菌、蜡样芽孢杆菌、莫氏变形杆菌、嗜酸微球菌。真菌种类有黄曲霉、烟曲霉、氧化孢镰刀菌、黄化青霉、黑曲霉、毛霉和酿酒酵母。堆肥样的pH值在5.8 ~ 6.9之间。氮、磷、钾含量随堆肥天数的增加而增加,重金属含量随堆肥天数的增加而减少。从第1天到第60天,3个样品的硫酸盐酶、磷酸酶、脱氢酶、淀粉酶和纤维素酶均呈上升趋势。3种酶中硫酸盐酶的含量最高,为25 ~ 76.5%,其次是磷酸酶(14 ~ 60.5%)、脱氢酶(20.5 ~ 55.0%)、纤维素酶(16.5 ~ 49%)和淀粉酶(5.0 ~ 38%)。
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