Journal of Natural Sciences Engineering and Technology最新文献

{"title":"ISOLATION METHODS FOR MOLECULAR DETECTION AND ANTIBIOTIC RESISTANCE PATTERN OF CAMPYLOBACTER SPP IN LAYER CHICKENS","authors":"O. Kehinde, M. Dipeolu, O. Awoyomi, M. Agbaje, O. Fasanmi, I. Kassem, O. Ojo, O. Adebowale, E. Omoshaba, J. Nwanta","doi":"10.51406/jnset.v19i1.2111","DOIUrl":"https://doi.org/10.51406/jnset.v19i1.2111","url":null,"abstract":"This study was conducted to compare two culture methods for the isolation of Campylobacter spp from commercial layer chickens and subsequently confirmed by Polymerase Chain Reaction assays (PCR). Furthermore, the antimicrobial resistance profiles of PCR positive Campylobacter isolates were determined.Cloacal swab samples (550) from chickens randomly selected from five poultry farms in the four geographical zones in Ogun State were cultured for Campylobacter using modified charcoal Cefoperazone deoxycholate agar (MCCDA) and an improved culture method involving Preston broth pre-enrichment and subsequent subculture on Mueller Hinton agar with Campylobacter growth supplements. Putative isolates were later confirmed by PCR assay and sequencing analysis.Other isolates that grew on MCCDA and confirmed by sequencing analysis are Enterococcus faecalis, Escherichis coli, Comamonas kerstli and Pseudomonas aeroginusa . The antibiotic resistant profile of all the isolates were evaluated genotypically for resistance genes to tetracyclines (tetO), multiclasses (cmeB), aminoglycosides (aphA-3-1) and β-lactams (Blaoxa-61) using multiplex PCR (mPCR), and phenotypically for chlortetracycline, tylosin, streptomycin, ciprofloxacin and erythromycin resistance by microbroth dilution method which correspond to the antibiotic resistance genes. The apparent prevalence of Campylobacter was 16.8% by MCCDA while none of the isolates was positive to PCR. Meanwhile, prevalence rate of 26% was obtained using Preston broth pre-enrichment and Mueller Hinton agar with Campylobacter growth supplements, of which 11/50 (22%) of the isolates was confirmed positive by PCR. Genotypic characterization of PCR positive isolates showed 10/11(90%) were C. coli, 1/11(10%) other Campylobacter species and 0% C. jejuni. All the isolates carried both tetO and cmeB resistant genes. The results of minimum inhibitory concentration presented all PCR positive isolates had resistance of 10/10(100%), 9/10(90%), 6/10(60%), 9/10(90%), and 8/10(80%) to tetracycline, ciprofloxacin, erythromycin, spectinomycin and tylosin respectively. In addition, all isolates carried multiple resistance to most antibiotics tested which are commonly used in poultry practice in Nigeria. Campylobacter spp in the study areas showed diverse genotypic characteristics, and gene mediated multidrug resistance. \u0000  \u0000 ","PeriodicalId":389500,"journal":{"name":"Journal of Natural Sciences Engineering and Technology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132494600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PATHOMORPHOLOGICAL CHANGES AND BACTERIAL PATHOGENS ASSOCIATED WITH SWINE PNEUMONIAS IN SOUTHWEST NIGERIA","authors":"M. Olaniyi, D. Ekum, E. Omoshaba, A. Sonibare, O. Ajayi, O. Ojo, I. Faramade, O. Mustapha, O. Alaka","doi":"10.51406/jnset.v19i1.2088","DOIUrl":"https://doi.org/10.51406/jnset.v19i1.2088","url":null,"abstract":"Pneumonia is a major economic threat to swine industry worldwide, however, there is still dearth of information on the pathology and associated pathogens in Nigeria, and these were therefore investigated. Lungs from 408 slaughtered pigs were randomly collected from abattoirs in Abeokuta, Ibadan and Lagos. The lung samples were cultured for bacterial pathogens using standard techniques, while formalin-fixed tissues were processed for histopathological examination. Grossly, the most consistent lesion was bronchopneumonia (35.3%). The main histopathological findings were lymphoid hyperplasia of bronchus-associated lymphoid tissue (BALT) (88.2%), suppurative bronchopneumonia (63.6%), suppurative bronchitis and bronchiolitis with concurrent epithelial hyperplasia (57.1%), as well as thickened alveolar septa due to cellular infiltration consisting predominantly of neutrophils (54.1%). Ten different species of bacteria were isolated from the lung samples in which two or more pathogens were isolated from each sample (82.7%). Pasteurella multocida was the most frequently isolated bacterium (54.8%). Among the bacteria isolated, there were significant (P < 0.05) differences in the frequencies of isolation of β-haemolytic Streptococci, P. multocida, Haemophilus species and Escherichia coli between the pneumonic lungs and apparently normal lungs. The results of bacterial culture, gross and histopathological changes recorded in this study are consistent with bacterial pneumonia possibly caused by most of the bacteria identified in the present study. \u0000  \u0000 ","PeriodicalId":389500,"journal":{"name":"Journal of Natural Sciences Engineering and Technology","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125205444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MYCOFLORA AND AFLATOXIN LEVELS IN WALNUT SAMPLES STORED IN DIFFERENT PACKAGING MATERIALS IN OGUN STATE","authors":"E. Oni, A. Aladesida, T. Obuotor, F. Alder, A. Badmos, Adeleye Tolulope Modupeola, A. Taiwo, A. J. Adeleke","doi":"10.51406/jnset.v19i1.2114","DOIUrl":"https://doi.org/10.51406/jnset.v19i1.2114","url":null,"abstract":"Aflatoxin levels in nuts and fruits pose a great threat to food storage and availability. This study aimed at determining the mycoflora and aflatoxin level in stored walnut under various conditions.  Freshly harvested walnuts were stored for 90 days  in three different media: jute bags, Plastic containers and sterile polythene bags under room (37ᵒC) and refrigeration (4ᵒC) temperature. After 90 days of storage, the stored walnuts were examined for fungal growth at one month interval after which colonial and morphological characterization were carried out to identify the fungi present. Proximate analysis and aflatoxin content of the walnut samples were determined by standard methods. Fungi isolated from the walnuts include; Aspergillus niger, Saccharomyces cerevisae, Penicillum notatum, Aspergillus sydowi, Fusarium oxysporium, and Rhizopus stolonifer. Walnuts stored in plastic container at room temperature had the lowest aflatoxin level of 0.002 ng/kg while that stored in polythene bags had the highest (0.054 ng/kg).  Proximate analysis also revealed that walnuts stored in polythene bag reduced significantly (p<0.05) in protein, ash content, fat, fibre, moisture and carbohydrates while samples stored in jute bag and plastic container remained unchanged. This study shows that storage methods contributed to the overall quality, shelf life and aflatoxin content of walnut and hence care should be taken during post harvesting process. \u0000  \u0000 ","PeriodicalId":389500,"journal":{"name":"Journal of Natural Sciences Engineering and Technology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132755708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IMPACT ASSESSMENT OF SUBSTANTIVE HYDRO-CLIMATIC VARIABLES ON 2012 FLOOD EXTENT IN YOLA AND ENVIRONS","authors":"A. Sadiq","doi":"10.51406/jnset.v19i1.2109","DOIUrl":"https://doi.org/10.51406/jnset.v19i1.2109","url":null,"abstract":"Flood is a seasonal phenomenon which is natural in it hazardous implication and occurs when there is relative high flow over the banks of the streams as a combine consequence of  high recorded data of hydro-climatic related variables in a given geographical area.  Yola North LGA, of Adamawa state had experienced an unprecedented flood in the year 2012 over the past decade which might have been influenced by some hydro-climatic variables and caused devastating effects on lives, properties, farmland and buildings respectively. This study focused on the impact assessment of substantive hydro-climatic variables on 2012 flood event in Yola -North and its environs. The hydro-climatic variables data were obtained from Meteorological station at UBRBDA, Yola for a decade. The amount of rainfall experienced was found to be highest (1085.2mm) in the year 2012 than any other year under consideration (2008-2017) except that of 2016, number of rainy days was highest (81 days) in the year 2012. Similarly, in the month of August in the year 2012 evaporation rate was lowest with about 69 mm than any other month of August in the decade, the annual value of water discharge was highest in the year 2012 over the decade with about 6,340(m3/s), the gauge height was found to be highest with about 7.33 m in the year 2012 and the water level was highest in the month of June, July and September  with the corresponding values of 3.37 m, 3.49 m and 6.58 m compared to similar months in the years of the decade respectively. These increased changes in some hydro-climatic data analyzed might be the fundamental natural factor that causes the unique flooding than any other factor in the year 2012 in the study area and over time posed negative impact on agricultural lands.  Therefore, the study recommends the urgent need to carry out a comprehensive seasonal hydro-climatic data record simulation analysis and variations with a view of taking them as a recipe and strategies of forecasting and predicting the reoccurrence of such phenomenon. The additional meteorological station should be provided by the government agencies in all agricultural zones of the state for adequate and wide range of hydro-climatic data recording for appropriate prediction of weather indices in future. \u0000  \u0000  \u0000 ","PeriodicalId":389500,"journal":{"name":"Journal of Natural Sciences Engineering and Technology","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131756365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"URBAN FLOOD IMPACTS, FLOOD WATER QUALITY AND RISK MAPPING OF OLODO AREA, IBADAN, NIGERIA","authors":"O. Adedeji, O. O. Olayinka, T. Ogundiran, O. O. Tope-Ajayi","doi":"10.51406/jnset.v19i1.2097","DOIUrl":"https://doi.org/10.51406/jnset.v19i1.2097","url":null,"abstract":"This study assessed urban flood impact, flood water quality and vulnerability around Olodo area of Ibadan region, Nigeria. The study employed remote sensing and GIS techniques in creating vulnerability and risk maps. Digital terrain model (DTM) was used to get the topography of the study area. Footprints of buildings along the Egberi riverbank and flood plain in Olodo were created in the GIS environment from high resolution satellite imagery. Buffering operation was conducted to classify the buildings into risk zones based on closeness to the riverbank using ArcGIS 10.0. The study revealed that 326 buildings were within the very vulnerable and vulnerable zones because they were less than 15.2m away from the riverbank. The characteristics of water quality change during the flood and non-flood periods. TSS, DO, NOD, and COD were all higher during the flood event. Microbial analysis showed that water quality levels in the floodwater exceeded water quality standards (e.g., the coliform excess from 10 to 10,000 times), and thus this may be a health risk for local people during flood events. Concentration of Escherichia coli (E. coli) ranged from 484 to 1290 cfu/100 mL during flooding compared to 192 to 295 cfu/100 mL after flood. Salmonella was found to be high ranging from 659 to 1840 cfu/100 mL during flooding compared to 530 to 1034 cfu/100 mL after flooding. \u0000  \u0000  \u0000 ","PeriodicalId":389500,"journal":{"name":"Journal of Natural Sciences Engineering and Technology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116750008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SPATIAL TECHNOLOGIES IN PASTURE AND RANGE MANAGEMENT: A REVIEW","authors":"J. Oyedepo, O. Onifade","doi":"10.51406/jnset.v19i1.2113","DOIUrl":"https://doi.org/10.51406/jnset.v19i1.2113","url":null,"abstract":"This paper looked at practical ways in which pasture and range management (P&RM) can benefit from application of spatial technologies; namely Satellite Remote Sensing, Global Positioning System and Geographical Information Science. Brief mention of these spatial technologies’ components and ways of their integrations (linear, interactive, hierarchical and complex models) were discussed with specific reference to P&RM. The paper also dwells on salient principles of applied remote sensing and geospatial technics in P&RM using examples and case studies revolving around rangeland management, spatial decision support and resource conservation. Specifically, the relevance of hyper spectral imageries and vegetation indices in cattle population and range roaming determination, grazing land and paddock site-specific management were demonstrated. It is hoped that the review will create awareness for the inclusion and use of remote sensing and geospatial technics in many areas of livestock management in Nigeria. \u0000  \u0000  \u0000 ","PeriodicalId":389500,"journal":{"name":"Journal of Natural Sciences Engineering and Technology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134128347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PREDICTING SOCIAL NETWORK ADDICTION USING VARIANT SIGMOID TRANSFER FEED-FORWARD NEURAL NETWORKS (FNN-SNA)","authors":"F. E. Ayo, O. Folorunso, A. Abayomi-Alli, A. Olubiyi","doi":"10.51406/jnset.v19i1.2110","DOIUrl":"https://doi.org/10.51406/jnset.v19i1.2110","url":null,"abstract":"Researchers have reflected on personal traits that may predict Social Networking Sites (SNS) addiction. However, most of the researchers involved in the findings of personality traits predictor for social networking addiction either postulate or based their conclusions on analytical tools. Moreso, a review of the literature reveals that the prediction of social networking addiction using classifiers have not been well researched. We examined the prediction of SNS addiction from a well-structured questionnaire consisting of sixteen (16) personality traits. The questionnaire was administered on the google form with a response rate of 95% out of the 102-sample size. Additionally, a three (3) variant sigmoid transfer feed- forward neural networks was developed for the prediction of SNS addiction. Result indicated that pertinence (β = 0.251, p  0.01) was the most powerful predictor of social networking addiction in general and less obscurity addiction (β = 0.244, p  0.01). Experimental results also showed that the developed classifier correctly predict SNS addiction with 98% accuracy compared to similar classifiers. \u0000  \u0000  \u0000 ","PeriodicalId":389500,"journal":{"name":"Journal of Natural Sciences Engineering and Technology","volume":"452 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125785411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AN ANALYSIS OF TRANSACTIONS IN E-PAYMENT SYSTEM USING MOBILE AGENTS","authors":"O. R. Vincent","doi":"10.51406/jnset.v19i1.2096","DOIUrl":"https://doi.org/10.51406/jnset.v19i1.2096","url":null,"abstract":"Commercial interactions between merchants and customers pose a significant concern as they are associated with a large volume of data and complex information, especially when there is a need for switching requirements. This paper presents an agent-based analysis of e-payment transactions with the switching operations. The model adopts an inter-bank transaction network and consists of a terminal point of sale (POI) and three essential players in e-payment: customer, bank (merchant), and the Switch. This study analyses the various payment interactions using agent technology. The agent coordinates movement while the negotiation protocol serves as an internal control of the payment agreements, while the interactive hosts are the platforms that determine the status of transactions. Each agent host is equipped with a Certification Authority (CA) to secure communication between the merchant and the customer. Different transactions that agents could make are examined with formal descriptions. The implementation is achieved in Jade and compares with the object serialization mechanism. The simulation results show higher quality adaptation of agent systems and evidence of agentisation of e-transaction with Switch. \u0000  \u0000  \u0000 ","PeriodicalId":389500,"journal":{"name":"Journal of Natural Sciences Engineering and Technology","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130303651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GEO-ELECTRIC INVESTIGATION OF GROUNDWATER POTENTIAL IN LADERIN HOUSING ESTATE, ABEOKUTA, SOUTHWESTERN, NIGERIA","authors":"A. Alabi, A. O. Adewale, O. O. Adeleke, F. G. Akinboro, Ayodeji Afe, A. Ganiyu","doi":"10.51406/jnset.v19i1.2098","DOIUrl":"https://doi.org/10.51406/jnset.v19i1.2098","url":null,"abstract":"Vertical Electrical Sounding (VES) and 2D Electrical Resistivity Tomography (ERT), with Schlumberger and Wenner electrode array configurations respectively were employed to investigate the groundwater potential of Laderin Housing Estate located at Oke-mosan, Abeokuta, Ogun state, southwestern Nigeria. The area is underlain by the basement complex of the southwestern Nigeria. The research aimed at determining the aquifer/groundwater zone and characterizes the lithology of the study area. The geophysical survey involving nine VES and four profile of 2-D ERT lines with lengths varying from 100 m to 150 m were carried out.  The field data from both the ERT and VES were processed and interpreted using RES2DINV and WINRESIST software respectively. Geometrical effects from the pseudo-section were removed and an image of true depth and true formation resistivity were produced. Three to four geo-electric layers were revealed in all, which are; topsoil, weathered layer, fractured and fresh basement rock. The result of 2 D inversion provide lithologic unit, weathering profile and geological structure favourable for groundwater potential. The results show that the basement rock was delineated with resistivity values that range between 701.3 Ωm and 9459.3 Ωm. The bedrock topography has a variable thickness of overburden between 3 m and above 16.4 m, which is fairly shallow.  The geophysical survey show that VES 2, VES 3, VES 4, VES 5, and VES 6 are possible location for groundwater extraction. The difficult terrain, where thick overburden are located are also promising target for groundwater development. \u0000  \u0000 ","PeriodicalId":389500,"journal":{"name":"Journal of Natural Sciences Engineering and Technology","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121210077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AN AUTOMATED ENERGY BILL METERING SYSTEM BASED ON GSM TECHNOLOGY","authors":"K. Okokpujie, A. Abayomi-Alli, O. Abayomi-Alli, M. Odusami, I. Okokpujie, O. A. Akinola","doi":"10.51406/jnset.v19i1.2093","DOIUrl":"https://doi.org/10.51406/jnset.v19i1.2093","url":null,"abstract":"The measurement of the energy consumed by residential and commercial buildings by utility provider is important in billing, control, and monitoring of the usage of energy. Traditional metering techniques used for the measurement of energy are not convenient and is prone to different forms of irregularities. These irregularities include meter failure, meter tampering, inaccuracies in billing due to human error, energy theft, and loss of revenue due to corruption, etc. This research study proposed the design and construction of a microcontroller-based electric energy metering system using the Global System for Mobile communication (GSM) network. This system provides a solution to the irregularities posed by the traditional metering technique by allowing the utility provider have access to remote monitoring capabilities, full control over consumer load, and remote power disconnection in the case of energy theft. Proteus simulation software was used to model the system hardware and the software was obtained by using embedded C programming and visual basic. It was observed that the system could remotely take accurate energy readings, provided full control over consumer loads and execute remote disconnection in case of energy theft. The system provides high performance and high accuracy in power monitoring and power management. \u0000  \u0000 ","PeriodicalId":389500,"journal":{"name":"Journal of Natural Sciences Engineering and Technology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128302480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}