Rice ferredoxin OsFd4 contributes to oxidative stress tolerance but compromises defense against blight bacteria

IF 6 1区 农林科学 Q1 AGRONOMY
Minfeng Lu , Jinhui Chen , Han Meng , Guangling Mo , Yunhong Liu , Fengping Chen , Zonghua Wang , Mo Wang
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引用次数: 0

Abstract

Ferredoxins (Fds) in plastids are the most upstream stromal electron receptors shuttling electrons to downstream metabolic systems and function in various physiological processes of dicots, but their roles in monocots’ response to stresses are still unclear. In this study, the functions of OsFd4, the major non-photosynthetic type Fd in rice, were characterized under oxidative stress and Xanthomonas oryzae pv. oryzae (Xoo) infection. OsFd4-knockout mutants displayed no defects in key agronomic traits and blast resistance, but were more sensitive to hydrogen peroxide (H2O2) treatment than the wild type. Transient expression of OsFd4 alleviated H2O2-induced rice cell death, suggesting that OsFd4 contributes to rice tolerance to exogenous oxidative stress. Deletion of OsFd4 enhanced rice immune responses against Xoo. OsFd4 formed a complex in vivo with itself and OsFd1, the major photosynthetic Fd in rice, and OsFd1 transcripts were increased in leaf and root tissues of the OsFd4-knockout mutants. These results indicate that OsFd4 is involved in regulating rice defense against stresses and interplays with OsFd1.

水稻铁氧还蛋白 OsFd4 有助于耐受氧化胁迫,但会削弱对枯萎病菌的防御能力
质体中的铁氧化还蛋白(Fds)是最上游的基质电子受体,将电子穿梭到下游代谢系统,在双子叶植物的各种生理过程中发挥作用,但它们在单子叶植物应对胁迫中的作用仍不清楚。本研究对水稻中主要的非光合型Fd--OsFd4在氧化胁迫和黄单胞菌(Xanthomonas oryzae pv. oryzae,Xoo)感染下的功能进行了表征。OsFd4 基因敲除突变体在关键农艺性状和抗稻瘟病方面没有表现出缺陷,但对过氧化氢(H2O2)处理比野生型更敏感。OsFd4的瞬时表达缓解了H2O2诱导的水稻细胞死亡,表明OsFd4有助于水稻耐受外源氧化胁迫。OsFd4的缺失增强了水稻对Xoo的免疫反应。OsFd4在体内与自身和OsFd1(水稻中主要的光合Fd)形成复合物,OsFd1转录本在OsFd4基因敲除突变体的叶片和根组织中增加。这些结果表明,OsFd4 参与调控水稻对胁迫的防御,并与 OsFd1 相互作用。
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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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